Nefm Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:137167 Tennese AA, et al., Loss of the Prader-Willi syndrome protein necdin causes defective migration, axonal outgrowth, and survival of embryonic sympathetic neurons. Dev Dyn. 2008 Jul;237(7):1935-43
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5582931
Gene symbol:	Nefm
Gene name:	neurofilament, medium polypeptide

Antibody:	anti-neurofilament clone 2H3
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

3A Merge ©

3A NF ©

Specimen 3A: embryonic day 18.5 (more

)
Note: Double labeled: green - Nefm; red - Casp3 (assay MGI:5582932).

Structure	Level	Pattern	Image	Note
TS26: superior cervical ganglion	Present		3A NF , 3A Merge

3A' Merge ©

3A' NF ©

Specimen 3A': embryonic day 18.5; Ndn^tm2Stw/Ndn^tm2Stw (more

)
Note: Double labeled: green - Nefm; red - Casp3 (assay MGI:5582932).

Structure	Level	Pattern	Image	Note
TS26: superior cervical ganglion	Present		3A' NF , 3A' Merge

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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