Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:148405
Nel-Themaat L, et al., Morphometric analysis of testis cord formation in Sox9-EGFP mice. Dev Dyn. 2009 May;238(5):1100-10
|
Assay type:
|
In situ reporter (knock in)
|
MGI Accession ID:
|
MGI:5581626
|
Gene symbol:
|
Sox9
|
Gene name:
|
SRY (sex determining region Y)-box 9
|
Reporter:
|
GFP
|
|
Direct Detection
|
|
Results
|
Specimen
1A:
embryonic day 11.5; Sox9tm1Haak/Sox9 +
(more )
Note: tail somite stage 19
Specimen
1A:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 11.5
|
Sex:
|
Male
|
Type:
|
Not Specified
|
Note:
|
tail somite stage 19
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: gonad |
Present |
Regionally restricted |
1A
|
Expressed in the Sertoli and germ cell mass. Surrounded by a layer of enhanced green fluorescent protein (EGFP)-negative tissue.
|
Specimen
1B:
embryonic day 12.5; Sox9tm1Haak/Sox9 +
(more )
Specimen
1B:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 12.5
|
Sex:
|
Male
|
Type:
|
Not Specified
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS20: germ cell of testis |
|
Strong |
Regionally restricted |
1B
|
Expressed along the entire length of the gonad. Expressed in the Sertoli and germ cell mass. Surrounded by a layer of enhanced green fluorescent protein (EGFP)-negative tissue.
|
TS20: testis |
|
Strong |
Regionally restricted |
1B
|
Expressed along the entire length of the gonad. Expressed in the Sertoli and germ cell mass. Surrounded by a layer of enhanced green fluorescent protein (EGFP)-negative tissue.
|
TS20: Sertoli cell |
Sertoli cell |
Strong |
Regionally restricted |
1B
|
|
Specimen
1C:
embryonic day 14.5; Sox9tm1Haak/Sox9 +
(more )
Specimen
1C:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Male
|
Type:
|
Not Specified
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: germ cell of testis |
|
Strong |
Regionally restricted |
1C
|
Expressed along the entire length of the gonad. Expressed in the Sertoli and germ cell mass. Surrounded by a layer of enhanced green fluorescent protein (EGFP)-negative tissue.
|
TS22: testis |
|
Strong |
Regionally restricted |
1C
|
Expressed along the entire length of the gonad. Expressed in the Sertoli and germ cell mass. Surrounded by a layer of enhanced green fluorescent protein (EGFP)-negative tissue.
|
TS22: Sertoli cell |
Sertoli cell |
Strong |
|
1C
|
|
Specimen
1D:
embryonic day 15.5; Sox9tm1Haak/Sox9 +
(more )
Specimen
1D:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 15.5
|
Sex:
|
Male
|
Type:
|
Not Specified
|
Specimen
1E:
embryonic day 16.5; Sox9tm1Haak/Sox9 +
(more )
Specimen
1E:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 16.5
|
Sex:
|
Male
|
Type:
|
Not Specified
|
Specimen
1F:
embryonic day 16.5; Sox9tm1Haak/Sox9 +
(more )
Specimen
1F:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 16.5
|
Sex:
|
Male
|
Type:
|
Not Specified
|
Specimen
2B/C/D:
embryonic day 13.5; Sox9tm1Haak/Sox9 +
(more )
Note: Double labeled: green - Sox9 reporter; red - Sox9 protein (assay MGI:5581710).
Specimen
2B/C/D:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Sox9 reporter; red - Sox9 protein (assay MGI:5581710).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS21: Sertoli cell |
Sertoli cell |
Present |
|
2B
,
2C/D
|
Expressed in the cytoplasm.
|
Specimen
2F/G/H:
embryonic day 13.5; Sox9tm1Haak/Sox9 +
(more )
Note: Double labeled: green - Sox9 reporter; red - Sox9 protein (assay MGI:5581710).
Specimen
2F/G/H:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Sox9 reporter; red - Sox9 protein (assay MGI:5581710).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS21: Sertoli cell |
Sertoli cell |
Present |
|
2F
,
2G/H
|
Expressed in the cytoplasm.
|
Specimen
4A:
embryonic day 11.5; Sox9tm1Haak/Sox9 +
(more )
Note: tail somite stage 19
Specimen
4A:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 11.5
|
Sex:
|
Male
|
Type:
|
Not Specified
|
Note:
|
tail somite stage 19
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: gonad |
Present |
Regionally restricted |
4A
|
Expressed in Sertoli cells.
|
Specimen
4B:
embryonic day 11.8; Sox9tm1Haak/Sox9 +
(more )
Note: tail somite stage 21
Specimen
4B:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 11.8
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Male
|
Type:
|
Not Specified
|
Note:
|
tail somite stage 21
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: gonad |
Present |
Regionally restricted |
4B
|
Expressed in Sertoli cells.
|
Specimen
4C:
embryonic day 12.1; Sox9tm1Haak/Sox9 +
(more )
Note: tail somite stage 24
Specimen
4C:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 12.1
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Male
|
Type:
|
Not Specified
|
Note:
|
tail somite stage 24
|
Specimen
4D:
embryonic day 12.5; Sox9tm1Haak/Sox9 +
(more )
Note: tail somite stage 32
Specimen
4D:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Male
|
Type:
|
Not Specified
|
Note:
|
tail somite stage 32
|
Specimen
4E:
embryonic day 14.5; Sox9tm1Haak/Sox9 +
(more )
Specimen
4E:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Male
|
Type:
|
Not Specified
|
Specimen
5B:
embryonic day 14.5; Sox9tm1Haak/Sox9 +
(more )
Specimen
5B:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Male
|
Type:
|
Not Specified
|
Specimen
5C:
embryonic day 14.5; Sox9tm1Haak/Sox9 +
(more )
Specimen
5C:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Male
|
Type:
|
Not Specified
|
Specimen
7A:
embryonic day 14.5; Sox9tm1Haak/Sox9 +
(more )
Specimen
7A:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Male
|
Type:
|
Not Specified
|
Specimen
7B:
embryonic day 14.5; Sox9tm1Haak/Sox9 +
(more )
Specimen
7B:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Male
|
Type:
|
Not Specified
|
Specimen
7C:
embryonic day 14.5; Sox9tm1Haak/Sox9 +
(more )
Specimen
7C:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Male
|
Type:
|
Not Specified
|
Specimen
7D:
embryonic day 14.5; Sox9tm1Haak/Sox9 +
(more )
Specimen
7D:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Male
|
Type:
|
Not Specified
|
Specimen
not shown:
embryonic day 10.5; Sox9tm1Haak/Sox9 +
(more )
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 10.5
|
Sex:
|
Male
|
Type:
|
Not Specified
|
Specimen
not shown:
embryonic day 10.5; Sox9tm1Haak/Sox9 +
(more )
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 10.5
|
Sex:
|
Female
|
Type:
|
Not Specified
|
Specimen
not shown:
embryonic day 12.0; Sox9tm1Haak/Sox9 +
(more )
Note: tail somite stage 22
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 12.0
|
Sex:
|
Male
|
Type:
|
Not Specified
|
Note:
|
tail somite stage 22
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: testis |
Strong |
Widespread |
|
Expressed along the entire length of the gonad.
|
Specimen
not shown:
embryonic day 12.5; Sox9tm1Haak/Sox9 +
(more )
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 12.5
|
Sex:
|
Female
|
Type:
|
Not Specified
|
Specimen
not shown:
embryonic day 14.5; Sox9tm1Haak/Sox9 +
(more )
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Female
|
Type:
|
Not Specified
|
Specimen
not shown:
embryonic day 15.5; Sox9tm1Haak/Sox9 +
(more )
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 15.5
|
Sex:
|
Female
|
Type:
|
Not Specified
|
Specimen
not shown:
embryonic day 16.5; Sox9tm1Haak/Sox9 +
(more )
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
embryonic day 16.5
|
Sex:
|
Female
|
Type:
|
Not Specified
|
Specimen
not shown:
postnatal adult; Sox9tm1Haak/Sox9 +
(more )
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S/SvEv * C57BL/6J * Swiss
|
Mutant Allele(s):
|
Sox9tm1Haak/Sox9+
|
Age:
|
postnatal adult
|
Sex:
|
Male
|
Type:
|
Not Specified
|
|