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Gene Expression Data
Assay Details
Assay
Reference: J:162870 Bax NA, et al., Cardiac malformations in Pdgfralpha mutant embryos are associated with increased expression of WT1 and Nkx2.5 in the second heart field. Dev Dyn. 2010 Aug;239(8):2307-17
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5576260
Gene symbol: Wt1
Gene name: WT1 transcription factor
Antibody: anti-WT1
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Results
Specimen 1c/d: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: proepicardium Present 1c , 1d
Specimen 1e/f: embryonic day 9.5; Pdgfratm1Sor/Pdgfratm1Sor (more )
Note: It is possible the specimen carries the Pdgfratm2Sor allele.
Structure Level Pattern Image Note
TS15: proepicardium Present 1f , 1e Reduced number of expressing cells, although the expression seemed more intense in the positive cells compared to the wildtype.
Specimen 3i: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: visceral pericardium Present 3i Expressed in epicardium.
TS20: heart mesentery Present 3i Expressed in migrating epicardium-derived cells.
Specimen 3j: embryonic day 12.5; Pdgfratm1Sor/Pdgfratm1Sor (more )
Note: It is possible the specimen carries the Pdgfratm2Sor allele.
Structure Level Pattern Image Note
TS20: visceral pericardium Present 3j Expressed in dissociated epicardium. There were no migrating epicardium-derived cells.
Specimen 3k: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: visceral pericardium Present 3k Expressed in epicardium.
TS21: heart mesentery Present 3k Expressed in migrating epicardium-derived cells.
Specimen 3l: embryonic day 13.5; Pdgfratm1Sor/Pdgfratm1Sor (more )
Note: It is possible the specimen carries the Pdgfratm2Sor allele.
Structure Level Pattern Image Note
TS21: visceral pericardium Present 3l Expressed in dissociated epicardium. There were no migrating epicardium-derived cells.
Specimen not shown: embryonic day 10.5; Pdgfratm1Sor/Pdgfratm1Sor (more )
Note: It is possible the specimen carries the Pdgfratm2Sor allele.
Structure Level Pattern Image Note
TS17: visceral pericardium Present Scattered Expressed in epicardium.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory