Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:165398
Barnes RM, et al., Analysis of the Hand1 cell lineage reveals novel contributions to cardiovascular, neural crest, extra-embryonic, and lateral mesoderm derivatives. Dev Dyn. 2010 Nov;239(11):3086-97
|
|
Assay type:
|
In situ reporter (knock in)
|
|
MGI Accession ID:
|
MGI:5574200
|
|
Gene symbol:
|
Hand1
|
|
Gene name:
|
heart and neural crest derivatives expressed 1
|
|
Reporter:
|
lacZ
|
|
Probe:
|
eGFPCRE probe
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
|
Results
|
Specimen
2C:
embryonic day 9.5; Hand1tm1Eno/Hand1 +
(more  )
Specimen
2C:
(close  )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2D:
embryonic day 10.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
2D:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2I:
embryonic day 9.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
2I:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
2J/P:
embryonic day 10.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
2J/P:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
4A:
embryonic day 12.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
4A:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4B:
embryonic day 12.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
4B:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: tongue |
Present |
Regionally restricted |
4B
|
Expressed in the midline.
|
Specimen
4C:
embryonic day 14.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
4C:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: tongue |
Present |
Regionally restricted |
4C
|
Expressed in the midline.
|
Specimen
4E:
embryonic day 12.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
4E:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
4F:
embryonic day 14.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
4F:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
4H:
embryonic day 10.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
4H:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4J:
embryonic day 18.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
4J:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4L:
embryonic day 12.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
4L:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
4M:
embryonic day 14.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
4M:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midgut |
Strong |
Regionally restricted |
4M
|
Expressed in the submucosa of the duodenum, a smooth muscle cell subpopulation surrounding the muscilaris externa, and the outer connective adventitia.
|
Specimen
4P:
embryonic day 12.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
4P:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: midgut mesenchyme |
Present |
Regionally restricted |
4P
|
Expressed in smooth muscle component.
|
Specimen
4Q:
embryonic day 14.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
4Q:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midgut |
Strong |
Regionally restricted |
4Q
|
Expressed in the submucosa of the duodenum, a smooth muscle cell subpopulation surrounding the muscilaris externa, and the outer connective adventitia.
|
Specimen
5A:
embryonic day 9.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
5A:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud mesenchyme |
Present |
Regionally restricted |
5A
|
Expressed in the lateral mesoderm underlying the forelimb bud.
|
Specimen
5C:
embryonic day 9.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
5C:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: hindlimb ridge mesenchyme |
Present |
Regionally restricted |
5C
|
Expressed in both the lateral mesoderm and the ventral mesenchyme of the limb bud.
|
Specimen
5E:
embryonic day 10.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
5E:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
5E
|
Expressed in the lateral mesoderm underlying the forelimb bud.
|
Specimen
5G:
embryonic day 10.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
5G:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5I:
embryonic day 12.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
5I:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5K:
embryonic day 12.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
5K:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5M:
embryonic day 14.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
5M:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: forelimb |
Present |
|
5M
|
Expression is downregulated.
|
Specimen
5O:
embryonic day 14.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
5O:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hindlimb |
Present |
|
5O
|
Expression is downregulated.
|
Specimen
7A:
embryonic day 9.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
7A:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk mesenchyme |
Present |
Regionally restricted |
7A
|
Expressed in lateral mesoderm.
|
Specimen
7B:
embryonic day 10.5; Hand1tm1Eno/Hand1 +
(more )
Specimen
7B:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk mesenchyme |
Present |
Regionally restricted |
7B
|
Expressed in lateral mesoderm.
|
Specimen
not shown:
postnatal adult; Hand1tm1Eno/Hand1 +
(more )
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Hand1tm1Eno/Hand1+
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Analysis Tools
