Gene Expression Data
Assay Details
Assay
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Reference:
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J:166847
Tonissoo T, et al., Nucleotide exchange factor RIC-8 is indispensable in mammalian early development. Dev Dyn. 2010 Dec;239(12):3404-15
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5571744
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
6M:
embryonic day 7.5
(more )
Specimen
6M:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6J
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6N:
embryonic day 8.5
(more )
Specimen
6N:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6J
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS13: embryo |
Present |
Regionally restricted |
6N
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Expression is in axial midline tissues.
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TS13: node |
Present |
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6N
|
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Specimen
6N':
embryonic day 8.5
(more )
Specimen
6N':
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6J
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Age:
|
embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Gelatin
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Structure |
Level |
Pattern |
Image |
Note |
TS13: embryo |
Present |
Regionally restricted |
6N'
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Expression is in axial midline tissues.
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TS13: node |
Present |
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6N'
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Specimen
6R:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6J
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Mutant Allele(s):
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Ric8atm1Aks/Ric8atm1Aks
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Age:
|
embryonic day 7.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
6S:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6J
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Mutant Allele(s):
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Ric8atm1Aks/Ric8atm1Aks
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: embryo |
Weak |
Regionally restricted |
6S
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Expression is in axial midline tissues.
|
TS13: node |
Weak |
|
6S
|
|
Specimen
6S':
(close )
Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6J
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Mutant Allele(s):
|
Ric8atm1Aks/Ric8atm1Aks
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Age:
|
embryonic day 8.5
|
Sex:
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Not Specified
|
Type:
|
section from whole mount
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Gelatin
|
|