Gene Expression Data
Assay Details
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Assay
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Reference:
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J:140470
Imuta Y, et al., Short limbs, cleft palate, and delayed formation of flat proliferative chondrocytes in mice with targeted disruption of a putative protein kinase gene, Pkdcc (AW548124). Dev Dyn. 2008 Dec 18;238(1):210-222
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5568852
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Gene symbol:
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Ihh
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Gene name:
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Indian hedgehog
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Probe:
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Ihh cDNA16
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
8J:
embryonic day 14.5
(more  )
Specimen
8J:
(close  )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
8J':
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Pkdcctm1Hssk/Pkdcctm1Hssk
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
8K:
embryonic day 15.5
(more )
Specimen
8K:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: humerus |
Present |
Regionally restricted |
8K
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Expression is in two domains of pre-hypertrophic chondrocytes.
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Specimen
8K':
embryonic day 15.5; Pkdcctm1Hssk/ Pkdcctm1Hssk
(more )
Note: K'' is a higher magnification image of the boxed are in K'.
Specimen
8K':
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Pkdcctm1Hssk/Pkdcctm1Hssk
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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K'' is a higher magnification image of the boxed are in K'.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: humerus |
Weak |
Regionally restricted |
8K''
,
8K'
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Expression is at the center.
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Specimen
8L:
embryonic day 16.5
(more )
Specimen
8L:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: humerus |
Present |
Regionally restricted |
8L
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Expression is in two domains of pre-hypertrophic chondrocytes.
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Specimen
8L':
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Pkdcctm1Hssk/Pkdcctm1Hssk
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: humerus |
Present |
Regionally restricted |
8L'
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Expression is in a large central domain.
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Analysis Tools