Gene Expression Data
Assay Details
Assay
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Reference:
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J:139010
MacDonald JL, et al., Histone deacetylases 1 and 2 are expressed at distinct stages of neuro-glial development. Dev Dyn. 2008 Aug;237(8):2256-67
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5566911
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Gene symbol:
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Nes
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Gene name:
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nestin
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Antibody:
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anti-nestin
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
1E:
embryonic day 13.5
(more )
Note: Double labeled: green - Nes; red - Hdac1 (assay MGI:5565614).
Specimen
1E:
(close )
Genetic Background:
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either: C57BL/6 or CD-1
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Nes; red - Hdac1 (assay MGI:5565614).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: telencephalon |
Present |
Regionally restricted |
1E
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Expressed in radial glia, which line the ventricles and extend process to the pial surface.
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Specimen
1K:
embryonic day 13.5
(more )
Note: Double labeled: green - Nes; red - Hdac1 (assay MGI:5565614).
Specimen
1K:
(close )
Genetic Background:
|
either: C57BL/6 or CD-1
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Age:
|
embryonic day 13.5
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
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Double labeled: green - Nes; red - Hdac1 (assay MGI:5565614).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: medulla oblongata |
Present |
Regionally restricted |
1K
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Expressed in radial glia in the ventricular zone around the 4th ventricle.
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Specimen
3I:
postnatal day 7
(more )
Note: Double labeled: green - Nes; red - Hdac1 (assay MGI:5565614).
Specimen
3I:
(close )
Genetic Background:
|
either: C57BL/6 or CD-1
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Age:
|
postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Double labeled: green - Nes; red - Hdac1 (assay MGI:5565614).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
Present |
|
3I
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Expressed in bergmann glia.
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Specimen
6C:
postnatal adult
(more )
Note: Double labeled: green - Nes; red - Hdac1 (assay MGI:5565614).
Specimen
6C:
(close )
Genetic Background:
|
either: C57BL/6 or CD-1
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Age:
|
postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
|
Note:
|
Double labeled: green - Nes; red - Hdac1 (assay MGI:5565614).
|
Specimen
6L:
postnatal adult
(more )
Note: Double labeled: green - Nes; red - Hdac1 (assay MGI:5565614).
Specimen
6L:
(close )
Genetic Background:
|
either: C57BL/6 or CD-1
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Age:
|
postnatal adult
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Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Nes; red - Hdac1 (assay MGI:5565614).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: telencephalon |
Present |
Regionally restricted |
6L
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Expressed in progenitors along the subventricular zone of the lateral ventricle.
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Specimen
6P:
postnatal adult
(more )
Note: Double labeled: green - Nes; red - Hdac2 (assay MGI:5565615).
Specimen
6P:
(close )
Genetic Background:
|
either: C57BL/6 or CD-1
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Age:
|
postnatal adult
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Nes; red - Hdac2 (assay MGI:5565615).
|
Specimen
6T:
postnatal adult
(more )
Note: Double labeled: green - Nes; red - Hdac2 (assay MGI:5565615).
Specimen
6T:
(close )
Genetic Background:
|
either: C57BL/6 or CD-1
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Nes; red - Hdac2 (assay MGI:5565615).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: telencephalon |
Present |
Regionally restricted |
6T
|
Expressed in progenitors along the subventricular zone of the lateral ventricle.
|
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