Probe:	S1pr3 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present		1J
TS13: somite	Present		1J
TS13: trunk unsegmented mesenchyme	Present		1J

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch	Present		2F
TS15: forelimb bud	Present		2F
TS15: head mesenchyme	Present		2F
TS15: somite	Present		2F
TS15: tail unsegmented mesenchyme	Present		2F

Structure	Level	Pattern	Image	Note
TS17: tail somite	Present		3H inset , 3H
TS17: tail unsegmented mesenchyme	Present	Regionally restricted	3H , 3H inset	Absent in the most anterior part of the presomitic mesoderm.
TS17: 1st branchial arch	Present		3H
TS17: head mesenchyme	Present		3H
TS17: limb	Present		3H
TS17: telencephalon	Present		3H
TS17: head	Present	Regionally restricted	3H	Expression is in the facial processes.

Structure	Level	Pattern	Image	Note
TS20: limb	Very strong		4H
TS20: somite	Very strong		4H
TS20: trunk mesenchyme	Very strong		4H
TS20: face	Very strong	Regionally restricted	4H	Expression is in the craniofacial region.
TS20: head	Very strong	Regionally restricted	4H	Expression is in the craniofacial region.

Structure	Level	Pattern	Image	Note
TS20: face	Present	Regionally restricted	9A	Expression is in the craniofacial region.
TS20: head	Present	Regionally restricted	9A	Expression is in the craniofacial region.
TS20: limb	Present		9A
TS20: somite	Present		9A
TS20: trunk mesenchyme	Present		9A

Structure	Level	Pattern	Image	Note
TS20: face	Present	Regionally restricted	9B	Expression is in the craniofacial region.
TS20: head	Present	Regionally restricted	9B	Expression is in the craniofacial region.
TS20: limb	Present		9B
TS20: somite	Present		9B
TS20: trunk mesenchyme	Present		9B

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present	Regionally restricted	9C	Expression is in the proximal region.
TS20: forelimb interdigital region	Present		9C

Structure	Level	Pattern	Image	Note
TS20: face	Present	Regionally restricted	9E	Expression is in the face, including the developing eyelid mesenchyme and the surrounding mesenchyme of the developing sinus hairs, ear, and jaw.
TS20: head mesenchyme	Present	Regionally restricted	9E	Expression is in the face, including the developing eyelid mesenchyme and the surrounding mesenchyme of the developing sinus hairs, ear, and jaw.
TS20: mandible primordium	Present		9E
TS20: nose	Present		9E
TS20: pharynx	Present		9E
TS20: tongue	Present		9E

Structure	Level	Pattern	Image	Note
TS20: mammary bud	Present		9F

Structure	Level	Pattern	Image	Note
TS20: tail somite	Present		9G

Structure	Level	Pattern	Image	Note
TS20: blood vessel	Present	Regionally restricted	9H	Expression is in intersegmental vessels.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	9I	Expression is in the neocortical ventricular zone and at two other sites.
TS20: head mesenchyme	Present	Regionally restricted	9I	Expression is in the periotic region.
TS20: mandible primordium	Present		9I
TS20: nose	Present		9I
TS20: pharynx	Present		9I
TS20: tongue	Present		9I

Structure	Level	Pattern	Image	Note
TS20: mandible primordium	Present		9J
TS20: nose	Present		9J
TS20: pharynx	Present		9J
TS20: tongue	Present		9J
TS20: head	Present	Regionally restricted	9J	Expression is surrounding the pituitary.

Structure	Level	Pattern	Image	Note
TS20: lung mesenchyme	Present		9K

Structure	Level	Pattern	Image	Note
TS20: genital tubercle	Present		9L

Structure	Level	Pattern	Image	Note
TS20: face	Present		10J
TS20: mandible primordium	Present		10J
TS20: nose	Present		10J
TS20: pharynx	Present		10J
TS20: tongue	Present		10J

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	10J'	Expression is in the neuroepithelium near the optic recess, and in the boundary region between the septum and thalamus.
TS20: head mesenchyme	Present	Regionally restricted	10J'	Expression is in the head mesenchyme surrounding the future pituitary.

Structure	Level	Pattern	Image	Note
TS19: branchial arch	Very strong		S2H
TS19: head mesenchyme	Very strong		S2H
TS19: limb	Very strong		S2H
TS19: trigeminal ganglion	Very strong		S2H
TS19: head	Very strong	Regionally restricted	S2H	Expression is in the facial processes.
TS19: forebrain	Present	Regionally restricted	S2H	Expression is at the border of the fore- and midbrain.
TS19: midbrain	Present	Regionally restricted	S2H	Expression is at the border of the fore- and midbrain.
TS19: somite	Very strong		S2H inset , S2H
TS19: tail unsegmented mesenchyme	Very strong		S2H inset , S2H

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Present	Regionally restricted	S3D	Expression is in the mesenchyme surrounding the eye.

Structure	Level	Pattern	Image	Note
TS20: limb interdigital region	Present		S3J

Structure	Level	Pattern	Image	Note
TS20: face	Present
TS20: forebrain	Present
TS20: genital tubercle	Present
TS20: limb	Present
TS20: lung mesenchyme	Present
TS20: mammary bud	Present
TS20: mandible primordium	Present
TS20: nose	Present
TS20: pituitary gland	Present
TS20: tail somite	Present
TS20: tongue	Present
TS20: trunk mesenchyme	Present
TS20: vibrissa mesenchyme	Present
TS20: head	Present	Regionally restricted		Expression is in the perioccular, perioptic region, and in sinus hairs.
TS20: blood vessel	Present	Regionally restricted		Expression is in intersegmental vessels.