Probe:	Lpar4 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS13: midbrain-hindbrain junction	Present		1F
TS13: future hindbrain	Present	Regionally restricted	1F	Expression is in the dorsal midline of the neural folds from the level of the rostral hindbrain to the forebrain.
TS13: future midbrain	Present	Regionally restricted	1F	Expression is in the dorsal midline of the neural folds from the level of the rostral hindbrain to the forebrain.
TS13: future prosencephalon	Present	Regionally restricted	1F	Expression is in the dorsal midline of the neural folds from the level of the rostral hindbrain to the forebrain.

Structure	Level	Pattern	Image	Note
TS13: midbrain-hindbrain junction	Present		1G
TS13: future hindbrain	Present	Regionally restricted	1G	Expression is in the dorsal midline of the neural folds from the level of the rostral hindbrain to the forebrain.
TS13: future midbrain	Present	Regionally restricted	1G	Expression is in the dorsal midline of the neural folds from the level of the rostral hindbrain to the forebrain.
TS13: future prosencephalon	Present	Regionally restricted	1G	Expression is in the dorsal midline of the neural folds from the level of the rostral hindbrain to the forebrain.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch	Present		2D
TS15: 1st branchial arch maxillary component	Present		2D
TS15: future hindbrain	Present		2D
TS15: midbrain-hindbrain junction	Present		2D

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present		3D
TS17: 2nd branchial arch	Present		3D
TS17: limb	Present		3D
TS17: liver	Present		3D
TS17: somite	Present		3D

Structure	Level	Pattern	Image	Note
TS20: limb	Present		4D
TS20: somite	Present		4D

Structure	Level	Pattern	Image	Note
TS20: limb	Present		6A
TS20: somite	Present		6A

Structure	Level	Pattern	Image	Note
TS20: limb	Present		6B
TS20: somite	Present		6B

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present	Regionally restricted	6C	Expression is in the proximal region.
TS20: forelimb digit	Present		6C

Structure	Level	Pattern	Image	Note
TS20: limb	Present		6E
TS20: nasal mesenchyme	Present		6E
TS20: neurohypophysis infundibulum	Present		6E
TS20: tongue	Present		6E
TS20: oral region	Present	Regionally restricted	6E	Expression is in tooth germ.

Structure	Level	Pattern	Image	Note
TS20: neurohypophysis infundibulum	Present		6F
TS20: nose	Present		6F
TS20: tongue	Present		6F

Structure	Level	Pattern	Image	Note
TS20: neurohypophysis infundibulum	Present		6G

Structure	Level	Pattern	Image	Note
TS20: somite	Present		6H	Somites in the caudal region have less expression.

Structure	Level	Pattern	Image	Note
TS20: nasal mesenchyme	Present		10D
TS20: tongue	Present		10D

Structure	Level	Pattern	Image	Note
TS20: nasal mesenchyme	Present		10D'
TS20: neurohypophysis infundibulum	Present		10D'
TS20: tongue	Present		10D'
TS20: mandibular process	Present	Regionally restricted	10D'	Expression is in tooth germ.

Structure	Level	Pattern	Image	Note
TS19: limb	Present	Regionally restricted	S2D	Expression is in the proximal region.

Structure	Level	Pattern	Image	Note
TS20: forelimb digit	Present	Regionally restricted	S3G	Expression is at the tips.
TS20: forearm	Present		S3G

Structure	Level	Pattern	Image	Note
TS20: face	Present
TS20: head	Present
TS20: limb	Present
TS20: neurohypophysis infundibulum	Present
TS20: somite	Present
TS20: tongue	Present
TS20: oral region	Present	Regionally restricted		Expression is in tooth germ.