Gene Expression Data
Assay Details
Assay
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Reference:
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J:139634
Peacock JD, et al., Temporal and spatial expression of collagens during murine atrioventricular heart valve development and maintenance. Dev Dyn. 2008 Sep 24;237(10):3051-3058
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5563225
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Gene symbol:
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Col12a1
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Gene name:
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collagen, type XII, alpha 1
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Antibody:
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anti-Collagen XII
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
2E/E':
embryonic day 17.5
(more )
Specimen
2E/E':
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS25: mitral valve leaflet |
Strong |
Regionally restricted |
2E'
,
2E
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Expressed more widely in proximal regions, with no expression in the distal tips.
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Specimen
3G:
postnatal day 1
(more )
Specimen
3G:
(close )
Genetic Background:
|
C57BL/6
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: mitral valve |
Present |
Regionally restricted |
3G
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Expressed throughout the mitral valve leaflets, with reduced expression at the distal tips.
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Specimen
4D:
postnatal month 2
(more )
Specimen
4D:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
postnatal month 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: mitral valve |
Present |
Regionally restricted |
4D
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Expressed along the ventricular surface and almost absent on the atrial surface the annulus. Low expression in the annular regions.
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