Sox2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:142645 Sherwood RI, et al., Transcriptional dynamics of endodermal organ formation. Dev Dyn. 2008 Dec 18;238(1):29-42
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5562906
Gene symbol:	Sox2
Gene name:	SRY (sex determining region Y)-box 2

Results

3C far right ©

3C Sox2 ©

Specimen 3C: embryonic day 9.5 (more

)
Note: Triple labeled: blue - Sox2; red - Cdx2 (assay MGI:5562893); green - Onecut1 (assay MGI:5562907).

Structure	Level	Pattern	Image	Note
TS15: embryo endoderm	Present		3C Sox2 , 3C far right	Expression is in the most anterior endodermal cells of the stomach-intestine border.
TS15: gut	Present		3C Sox2 , 3C far right	Expression is in the most anterior endodermal cells of the stomach-intestine border.

3D far right ©

3D Sox2 ©

Specimen 3D: embryonic day 9.5 (more

)
Note: Triple labeled: blue - Sox2; red - Cdx2 (assay MGI:5562893); green - Pdx1 (assay MGI:5562896).

Structure	Level	Pattern	Image	Note
TS15: embryo endoderm	Present	Regionally restricted	3D Sox2 , 3D far right	Expression is in the most anterior endodermal cells of the stomach-intestine border.
TS15: gut	Present	Regionally restricted	3D Sox2 , 3D far right	Expression is in the most anterior endodermal cells of the stomach-intestine border.

Specimen not shown: embryonic day 7.75 (more

)

Structure	Level	Pattern	Image	Note
TS12: gut endoderm	Present	Regionally restricted		Expression is in the most anterior and posterior cells.

Specimen 4A: embryonic day 8.25 (more

)
Note: 4-6 somites. Triple labeled: green - Sox2; red - Cdx2 (assay MGI:5562893); blue - Foxa2 (assay MGI:5562658).

Structure	Level	Pattern	Image	Note
TS13: gut endoderm	Present	Regionally restricted	4A left panel , 4A right panel	Expression extends posteriorly at the stomach-intestine border.

4B ©

Specimen 4B: embryonic day 8.5 (more

)
Note: 9-11 somites. Double labeled: green - Sox2; red - Cdx2 (assay MGI:5562893).

Structure	Level	Pattern	Image	Note
TS13: gut endoderm	Present	Regionally restricted	4B	Expression extends posteriorly at the stomach-intestine border.

Specimen 4C: embryonic day 8.75 (more

)
Note: 13-15 somites. Triple labeled: green - Sox2; red - Cdx2 (assay MGI:5562893); blue - Pdx1 (assay MGI:5562896).

Structure	Level	Pattern	Image	Note
TS14: gut	Present	Regionally restricted	4C left panel , 4C right panel	Expression extends to the foregut-midgut junction.

Specimen 4H: embryonic day 8.5 (more

)
Note: 6-8 somites. Triple labeled: green - Sox2; red - Cdx2 (assay MGI:5562893); blue - Onecut1 (assay MGI:5562907).

Structure	Level	Pattern	Image	Note
TS13: gut	Present	Regionally restricted	4H right panel

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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