Gene Expression Data
Assay Details
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Assay
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Reference:
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J:142102
Vincentz JW, et al., Cooperative interaction of Nkx2.5 and Mef2c transcription factors during heart development. Dev Dyn. 2008 Dec;237(12):3809-19
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5562555
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Gene symbol:
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Isl1
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Gene name:
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ISL1 transcription factor, LIM/homeodomain
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Probe:
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Isl1 probe21
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
5C:
embryonic day 9.5
(more )
Note: 20-22 somites.
Specimen
5C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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20-22 somites.
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Specimen
5F:
embryonic day 9.5
(more )
Note: 20-22 somites.
Specimen
5F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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20-22 somites.
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Specimen
5I:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Nkx2-5tm1Rph/Nkx2-5tm1Rph
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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20-22 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: heart |
Present |
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5I
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Expression in this mutant is expanded compared to wild-type.
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Specimen
5L:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Mef2ctm1Eno/Mef2ctm1Eno
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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20-22 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: heart |
Present |
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5L
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Expression in this mutant is the same as in wild-type.
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Specimen
5O:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Mef2ctm1Eno/Mef2ctm1Eno Nkx2-5tm1Rph/Nkx2-5tm1Rph
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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20-22 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: heart |
Present |
Regionally restricted |
5O
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Expression is uniform across the inner curvature of the heart.
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