Probe:	MGC clone 30527
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Weak		1A

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent		1A inset	Expression was not consistently seen among the youngest embryos at early headfold stage.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Strong		1B	Expression in mesodermal cells intensified at the late headfold stage.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Strong	Regionally restricted	1B inset	Expression was strong in the lateral mesodermal wings, which extended at lower levels toward the base of the headfolds and was excluded from the primitve streak and node.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	1C	Expression was regionalized from the base of the headfolds to the node region.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Non-Uniform	1D	Expression was in a striped pattern rostrally to the node.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present		1D inset
TS11: neural ectoderm	Absent		1D inset
TS11: node	Absent		1D inset
TS11: embryo ectoderm	Absent		1D inset	No signal was seen in the epiblast.

Structure	Level	Pattern	Image	Note
TS12: future midbrain	Weak		1E
TS12: embryo	Strong	Regionally restricted	1E	Strong expression was found in the prospective posterior cervical and trunk levels, but many head tissues and the caudal region of the embryo were devoid of transcripts.

Structure	Level	Pattern	Image	Note
TS12: future midbrain	Weak		1E upper inset

Structure	Level	Pattern	Image	Note
TS12: sinus venosus	Present		1E lower inset

Structure	Level	Pattern	Image	Note
TS12: somite	Strong		1F
TS12: future hindbrain floor plate	Present	Regionally restricted	1F	A stripe of expression was noted in the floorplate of the posterior hindbrain.
TS12: embryo	Strong	Regionally restricted	1F	Strong expression was found in the prospective posterior cervical and trunk levels, but many head tissues were devoid of transcripts.

Structure	Level	Pattern	Image	Note
TS12: somite	Present	Regionally restricted	1G	As somitic differentiation proceeded, expression became concentrated dorsally.
TS12: lateral plate mesoderm	Strong	Regionally restricted	1G	Dorsally to the heart, signal was found in lateral mesoderm up to the level of the posterior branchial region, surrounding the foregut pocket.
TS12: future midbrain	Present		1G
TS12: allantois	Weak	Regionally restricted	1G	Weak expression was detected in a patch of cells at the base of the allantois.
TS12: embryo	Strong	Regionally restricted	1G	Strong expression was found in the prospective posterior cervical and trunk levels, but many head tissues and the caudal region of the embryo were devoid of transcripts.

Structure	Level	Pattern	Image	Note
TS12: allantois	Weak	Regionally restricted	1G inset	Weak expression was detected in a patch of cells at the base of the allantois.

Structure	Level	Pattern	Image	Note
TS12: somite	Present	Regionally restricted	1H	In the posterior region of the embryo, expression was detected in the newly formed somites undergoing epithelialization.
TS12: trunk unsegmented mesenchyme	Absent		1H
TS12: lateral plate mesoderm	Present	Regionally restricted	1H	Expression extended more caudally in the lateral plate mesoderm than the somitic mesoderm.
TS12: neural tube floor plate	Present		1H
TS12: embryo	Strong	Regionally restricted	1H	Strong expression was found in the prospective posterior cervical and trunk levels, but the caudal region of the embryo was devoid of transcripts.

Structure	Level	Pattern	Image	Note
TS13: somite	Present	Regionally restricted	1I	As somitic differentiation proceeded, expression became concentrated dorsally.
TS13: neural tube floor plate	Present		1I

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Strong	Regionally restricted	1J	Dorsally to the heart, signal was found in lateral mesoderm up to the level of the posterior branchial region, surrounding the foregut pocket.
TS13: foregut diverticulum	Present	Single cells	1J	A few cells of the lateral foregut epithelium were positive.
TS13: neural tube floor plate	Present		1J

Structure	Level	Pattern	Image	Note
TS13: somite	Present	Regionally restricted	1K	As somitic differentiation proceeded, expression became concentrated dorsally.
TS13: lateral plate mesoderm	Strong	Regionally restricted	1K	Dorsally to the heart, signal was found in lateral mesoderm up to the level of the posterior branchial region, surrounding the foregut pocket.
TS13: neural tube floor plate	Present		1K

Structure	Level	Pattern	Image	Note
TS13: somite	Present	Regionally restricted	1L	As somitic differentiation proceeded, expression became concentrated dorsally.
TS13: intermediate mesenchyme	Present		1L
TS13: lateral plate mesoderm	Present		1L
TS13: neural tube floor plate	Present		1L

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Weak		2A
TS15: midbrain-hindbrain junction	Weak		2A
TS15: somite	Present	Regionally restricted	2A , 2C	Expression was detected within differentiating somites. The somites posterior to the forelimb level were only weakly labeled.
TS15: foregut	Present	Regionally restricted	2A , 2B	Expression was concentrated around the foregut in the posterior pharyngeal region.
TS15: trunk mesenchyme	Strong	Regionally restricted	2A	Expression was detected in intermediate and lateral plate mesoderm from forelimb to allantoic levels.
TS15: nephric duct	Strong	Regionally restricted	2C , 2A	Highest expression was specific to the mesonephric ducts within the intermediate mesoderm.
TS15: olfactory placode	Absent		2A	Olfactory placodal epithelium was not labeled.

Structure	Level	Pattern	Image	Note
TS15: midbrain-hindbrain junction	Weak		2A upper inset

Structure	Level	Pattern	Image	Note
TS15: midbrain-hindbrain junction	Weak		2A lower inset

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present	Regionally restricted	2D	Expression was detected along the entire proximal margin of the forelimb bud. Expressing cells extended along both the anterior and posterior margins, and labeling was most intense in the zone of polarizing activity (bracket).

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present	Regionally restricted	2E	Expression was detected along the entire proximal margin of the forelimb bud. Expressing cells extended along both the anterior and posterior margins, and labeling was most intense in the zone of polarizing activity (bracket).

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present	Regionally restricted	2F	Expression was detected in dorsal superficial mesenchyme at the level of the posterior hindbrain.
TS15: foregut	Present	Regionally restricted	2F	Expression was detected in foregut endoderm, including at the level of the second branchial arch.
TS15: future hindbrain floor plate	Present		2F

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present	Regionally restricted	2G	Expression was detected in dorsal superficial mesenchyme at the level of the posterior hindbrain.
TS15: foregut	Present	Regionally restricted	2G	Expression was detected in foregut endoderm.

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present	Regionally restricted	2H	Expression was detected in dorsal superficial mesenchyme at the level of the posterior hindbrain.
TS15: trunk mesenchyme	Present	Regionally restricted	2H	Expression was highest in the mesenchyme surrounding the foregut and dorsal aortae, dorsally to the atrial chamber of the developing heart.
TS15: foregut	Present	Regionally restricted	2H	Expression was detected in foregut endoderm.

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present	Regionally restricted	2I	Expression was detected in dorsal superficial mesenchyme at the level of the posterior hindbrain.
TS15: trunk mesenchyme	Present	Regionally restricted	2I	Expression was highest in the mesenchyme surrounding the foregut and dorsal aortae, dorsally to the atrial chamber of the developing heart.
TS15: foregut	Present	Regionally restricted	2I	Expression was detected in foregut endoderm.

Structure	Level	Pattern	Image	Note
TS15: optic vesicle	Present	Regionally restricted	2J	Expression was dorsally restricted.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present	Regionally restricted	2K	Expression was restricted to cells along the hindbrain surface.

Structure	Level	Pattern	Image	Note
TS15: foregut	Present	Regionally restricted	2L	At more posterior levels, endoderm-specific expression was detected at the prospective stomach level.

Structure	Level	Pattern	Image	Note
TS15: dermomyotome	Present	Regionally restricted	2M	Expression was detected in the dorsal edge of the dermomyotome.
TS15: trunk mesenchyme	Present	Regionally restricted	2M	Expression was detected in cells surrounding the dorsal spinal cord and in the lateral plate mesoderm somatopleure layer.
TS15: nephric duct	Strong	Regionally restricted	2M	Highest expression was specific to the mesonephric ducts within the intermediate mesoderm.
TS15: neural tube floor plate	Present	Regionally restricted	2M	Expression was detected in the floor plate of the posterior spinal cord.

Structure	Level	Pattern	Image	Note
TS15: trunk mesenchyme	Present	Regionally restricted	2M inset	Expression was detected in the lateral plate mesoderm splanchnopleure layer.

Structure	Level	Pattern	Image	Note
TS15: nephric duct	Strong	Regionally restricted	2N	Highest expression was specific to the mesonephric ducts within the intermediate mesoderm.

Structure	Level	Pattern	Image	Note
TS17: branchial pouch endoderm	Present		3A
TS17: branchial arch ectoderm	Present	Regionally restricted	3A	Expression was detected in the ectodermal component of the branchial pouches.

Structure	Level	Pattern	Image	Note
TS17: branchial pouch endoderm	Present		3B
TS17: branchial arch ectoderm	Present	Regionally restricted	3B	Expression was detected in the ectodermal component of the branchial pouches.

Structure	Level	Pattern	Image	Note
TS17: branchial pouch endoderm	Present		3C
TS17: branchial arch ectoderm	Present	Regionally restricted	3C	Expression was detected in the ectodermal component of the branchial pouches.

Structure	Level	Pattern	Image	Note
TS17: olfactory placode	Present			Expression in olfactory placodal epithelium.

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Present	Regionally restricted	3D	Most of the nasal placodal epithelium was negative.
TS20: latero-nasal process mesenchyme	Present	Regionally restricted	3D	Expression was detected in the distal mesenchyme of the growing lateral nasal processes.

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Strong	Regionally restricted	3E	Strong expression was found distally at the level of the nasal pits.

Structure	Level	Pattern	Image	Note
TS22: nasal cavity epithelium	Strong	Regionally restricted	3F	Highest levels of olfactory epithelium expression were detected toward the recesses of the developing olfactory cavities.

Structure	Level	Pattern	Image	Note
TS22: nasal cavity epithelium	Strong	Regionally restricted	3G	Highest levels of olfactory epithelium expression were detected toward the recesses of the developing olfactory cavities.
TS22: nasal mesenchyme	Present	Regionally restricted	3G	Expression was detected toward the distal tips of both the lateral and medial nasal processes.

Structure	Level	Pattern	Image	Note
TS20: thyroid gland	Present	Regionally restricted	3H	Expression was detected in cells surrounding the developing thyroid duct.

Structure	Level	Pattern	Image	Note
TS22: tongue	Present	Regionally restricted	3I	Expression was detected at the base of the tongue.
TS22: dental papilla	Present		3I
TS22: dental sac	Present		3I

Structure	Level	Pattern	Image	Note
TS24: dental papilla	Present
TS24: dental sac	Present

Structure	Level	Pattern	Image	Note
TS24: nasal cavity epithelium	Strong	Regionally restricted	3J	Expression was decreased in the olfactory epithelium by E16.5, but it was strongly induced in cells of the developing nasal serous glands.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf epithelium	Strong	Regionally restricted	3K	Highest expression was detected in the palatal epithelium near the area of fusion of the palatal shelves.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf mesenchyme	Present	Regionally restricted	3L	Expression was detected toward the extremities of the palatal mesenchyme in regions where the palatal shelves had not yet fused.
TS22: palatal shelf epithelium	Strong	Regionally restricted	3L	Highest expression was detected in the palatal epithelium near the area of fusion of the palatal shelves.

Structure	Level	Pattern	Image	Note
TS22: mandible	Present	Regionally restricted	3M	Expression was detected toward the extremities of the maxillary and mandibular processes.
TS22: maxilla	Present	Regionally restricted	3M	Expression was detected toward the extremities of the maxillary and mandibular processes.

Structure	Level	Pattern	Image	Note
TS22: mandible	Present	Regionally restricted	3N	Expression was detected toward the extremities of the maxillary and mandibular processes.
TS22: maxilla	Present	Regionally restricted	3N	Expression was detected toward the extremities of the maxillary and mandibular processes.

Structure	Level	Pattern	Image	Note
TS26: face	Weak			Many of the facial regions or structures that showed expression at fetal stages were weakly or no longer labeled by E18.5

Structure	Level	Pattern	Image	Note
TS26: dental papilla	Present		3O

Structure	Level	Pattern	Image	Note
TS26: dental papilla	Present		3P
TS26: molar mesenchyme	Present	Regionally restricted	3P	Expression was concentrated toward the tips of the cusps facing the inner dental epithelium.

Structure	Level	Pattern	Image	Note
TS20: pleura	Strong		4A
TS20: pericardium	Ambiguous		4A	There was no (or comparatively much weaker) labeling of the pericardium.

Structure	Level	Pattern	Image	Note
TS22: pleura	Present		4B	Expression persisted at lower levels in the pleural mesothelium.
TS22: bronchus mesenchyme	Present	Regionally restricted	4B	Expression was detected in the proximal bronchi mesenchyme.
TS22: diaphragm	Weak	Regionally restricted	4B	Weak expression was detected in lateral regions of the prospective diaphragm.

Structure	Level	Pattern	Image	Note
TS22: pericardium	Weak	Regionally restricted	4B inset	No expression was detected within heart tissues, except for weak signal in some regions of the epicardium.

Structure	Level	Pattern	Image	Note
TS22: pleura	Present		4C	Expression persisted at lower levels in the pleural mesothelium.
TS22: bronchus mesenchyme	Present	Regionally restricted	4C	Expression was detected in the proximal bronchi mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: thymus primordium	Present	Patchy	4D

Structure	Level	Pattern	Image	Note
TS22: spleen primordium	Present	Patchy	4E
TS22: stomach epithelium	Absent		4E
TS22: stomach mesenchyme	Present		4E

Structure	Level	Pattern	Image	Note
TS24: intestine	Present	Regionally restricted	4F	Expression was localized to two concentric layers.

Structure	Level	Pattern	Image	Note
TS17: mesonephric tubule	Present	Regionally restricted	4G	Expression was detected in the segmented mesonephric tubules.

Structure	Level	Pattern	Image	Note
TS22: renal cortex	Present	Regionally restricted	4H	Expression was concentrated in the outer cortical region.

Structure	Level	Pattern	Image	Note
TS22: rectum	Present	Regionally restricted	4I	Expression was localized to two concentric layers.
TS22: nephric duct	Present	Regionally restricted	4I	Expression was detected around the distal portion of the Wolffian duct.
TS22: paramesonephric duct	Present	Regionally restricted	4I	Expression was detected around the distal portion of the Mullerian duct.

Structure	Level	Pattern	Image	Note
TS24: uterine horn	Present
TS24: vagina	Present

Structure	Level	Pattern	Image	Note
TS24: rectum	Present	Regionally restricted	4J	Expression was localized to two concentric layers.
TS24: urethra of male	Present	Regionally restricted	4J	Expression was detected in the urethral mesenchyme.
TS24: peri-prostatic mesenchyme	Present	Regionally restricted	4J

Structure	Level	Pattern	Image	Note
TS20: intestine mesenchyme	Present		4K
TS20: genital tubercle	Present	Regionally restricted	4K	Expression was detected at the base of the genital tubercle.

Structure	Level	Pattern	Image	Note
TS20: limb interdigital region mesenchyme	Present		4L

Structure	Level	Pattern	Image	Note
TS22: limb interdigital region mesenchyme	Strong		4M

Structure	Level	Pattern	Image	Note
TS24: limb interdigital region mesenchyme	Present