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Gene Expression Data
Assay Details
Assay
Reference: J:163739 Leung AW, et al., Loss of procollagen IIA from the anterior mesendoderm disrupts the development of mouse embryonic forebrain. Dev Dyn. 2010 Sep;239(9):2319-29
Assay type: RNA in situ
MGI Accession ID: MGI:5554522
Gene symbol: Shh
Gene name: sonic hedgehog
Probe: Shh cDNA2
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 4A +/+ 0s: embryonic day 8.0 (more )
Note: Headfold stage.
Structure Level Pattern Image Note
TS11: mesendoderm Present Regionally restricted 4A 0s +/+ Expression was detected in the anterior mesendoderm.
Specimen 4A -/- 0s: embryonic day 8.0; Col2a1tm1.1Ksec/Col2a1tm1.1Ksec (more )
Note: Headfold stage.
Structure Level Pattern Image Note
TS11: mesendoderm Weak Regionally restricted 4A 0s -/- Expression was reduced in the prechordal anterior mesendoderm.
Specimen 4A +/- 4s: embryonic day 8.0; Col2a1tm1.1Ksec/Col2a1+ (more )
Note: 4 somites.
Structure Level Pattern Image Note
TS12: embryo Present Regionally restricted 4A 4s +/- Expression was detected in the prechordal anterior mesendoderm.
Specimen 4A -/- 4s: embryonic day 8.0; Col2a1tm1.1Ksec/Col2a1tm1.1Ksec (more )
Note: 4 somites.
Structure Level Pattern Image Note
TS12: embryo Present Regionally restricted 4A 4s -/- Expression was reduced in the prechordal anterior mesendoderm.
Specimen 4C +/- left: embryonic day 8.5; Col2a1tm1.1Ksec/Col2a1+ (more )
Structure Level Pattern Image Note
TS13: embryo Present Regionally restricted 4C +/- left Expression was detected in the remaining anterior mesendoderm.
TS13: notochordal plate Present 4C +/- left
TS13: notochord Present Regionally restricted 4C +/- left Expression was detected in the anterior notochord.
Specimen 4C +/- right: embryonic day 8.5; Col2a1tm1.1Ksec/Col2a1+ (more )
Structure Level Pattern Image Note
TS13: embryo Present Regionally restricted 4C +/- right Expression was detected in the remaining anterior mesendoderm.
TS13: notochordal plate Present 4C +/- right
TS13: notochord Present Regionally restricted 4C +/- right Expression was detected in the anterior notochord.
Specimen 4i: embryonic day 8.5; Col2a1tm1.1Ksec/Col2a1+ (more )
Structure Level Pattern Image Note
TS13: notochordal plate Present 4i
Specimen 4ii: embryonic day 8.5; Col2a1tm1.1Ksec/Col2a1+ (more )
Structure Level Pattern Image Note
TS13: notochord Present Regionally restricted 4ii Expression was detected in the anterior notochord.
Specimen 4C -/- left: embryonic day 8.5; Col2a1tm1.1Ksec/Col2a1tm1.1Ksec (more )
Structure Level Pattern Image Note
TS13: embryo Present Regionally restricted 4C -/- left Expression was detected in the remaining anterior mesendoderm.
TS13: notochordal plate Weak 4C -/- left There was reduced expression in the prechordal plate of the mutant.
TS13: notochord Present Regionally restricted 4C -/- left Normal expression was detected in the anterior notochord.
Specimen 4C -/- right: embryonic day 8.5; Col2a1tm1.1Ksec/Col2a1tm1.1Ksec (more )
Structure Level Pattern Image Note
TS13: embryo Present Regionally restricted 4C -/- right Expression was detected in the remaining anterior mesendoderm.
TS13: notochordal plate Weak 4C -/- right There was reduced expression in the prechordal plate of the mutant.
TS13: notochord Present Regionally restricted 4C -/- right Normal expression was detected in the anterior notochord.
Specimen 4iii: embryonic day 8.5; Col2a1tm1.1Ksec/Col2a1tm1.1Ksec (more )
Structure Level Pattern Image Note
TS13: notochordal plate Weak 4iii There was reduced expression in the prechordal plate of the mutant.
Specimen 4iv: embryonic day 8.5; Col2a1tm1.1Ksec/Col2a1tm1.1Ksec (more )
Structure Level Pattern Image Note
TS13: notochord Present Regionally restricted 4iv Normal expression was detected in the anterior notochord.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
MGI 6.24 		The Jackson Laboratory