Gene Expression Data
Assay Details
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Assay
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Reference:
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J:169913
Hudson LD, et al., A tool for examining the role of the zinc finger myelin transcription factor 1 (Myt1) in neural development: Myt1 knock-in mice. Transgenic Res. 2011 Aug;20(4):951-61
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5553348
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Gene symbol:
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Rbfox3
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Gene name:
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RNA binding protein, fox-1 homolog (C. elegans) 3
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Antibody:
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Anti-NeuN (clone A60; MAB377)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to Alexa Fluor 594
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Assay notes:
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The fixative included picric acid.
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Results
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Specimen
4A/B +/+:
embryonic day 18.0
(more  )
Note: Double labeled: red - Rbfox3; green - Myt1 reporter (assay MGI:5553340).
Specimen
4A/B +/+:
(close  )
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Genetic Background:
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involves: 129/SvEv * C57BL/6
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Rbfox3; green - Myt1 reporter (assay MGI:5553340).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: spinal cord |
Present |
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4B +/+
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4A +/+
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Expression is present in differentiated neurons.
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Specimen
4A/B +/-:
(close )
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Genetic Background:
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B6.129S-Myt1tm1(EGFP/icre)Ldh
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Mutant Allele(s):
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Myt1tm1(EGFP/icre)Ldh/Myt1+
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Rbfox3; green - Myt1 reporter (assay MGI:5553340).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: spinal cord |
Present |
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4B +/-
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4A +/-
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Expression is present in differentiated neurons. Based on the quantification presented in Fig. 6, the number of labeled cells present in heterozygotes is similar to that shown for wild-type mice.
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Specimen
4A/B -/-:
(close )
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Genetic Background:
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B6.129S-Myt1tm1(EGFP/icre)Ldh
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Mutant Allele(s):
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Myt1tm1(EGFP/icre)Ldh/Myt1tm1(EGFP/icre)Ldh
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Rbfox3; green - Myt1 reporter (assay MGI:5553340).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: spinal cord |
Present |
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4A -/-
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4B -/-
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Expression is present in differentiated neurons. Based on the quantification presented in Fig. 6, the number of labeled cells present in homozygotes is similar to that shown for wild-type mice.
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Specimen
5:
embryonic day 18.0; Myt1tm1(EGFP/icre)Ldh/0
(more )
Note: Double labeled: red - Rbfox3; green - Myt1 reporter (assay MGI:5553340). The legend refers to this specimen as homozygotic, while the text says it is heterozygotic.
Specimen
5:
(close )
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Genetic Background:
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B6.129S-Myt1tm1(EGFP/icre)Ldh
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Mutant Allele(s):
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Myt1tm1(EGFP/icre)Ldh/0
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Rbfox3; green - Myt1 reporter (assay MGI:5553340). The legend refers to this specimen as homozygotic, while the text says it is heterozygotic.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: spinal cord |
Present |
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5
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Expression is present in differentiated neurons.
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