Antibody:	Anti-LYVE-1
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS21: lymphatic system	Present	Regionally restricted	2A , 2B	Expression was detected in the jugular lymph sacs, including the extensions.
TS21: mediastinum	Present	Scattered	2B , 2A
TS21: dermis	Present	Scattered	2A
TS21: meninges	Present	Scattered	2A

Structure	Level	Pattern	Image	Note
TS20: lymphatic system	Present	Regionally restricted	2C	Expression was detected in the jugular lymphatic sacs.
TS20: mediastinum	Present	Scattered	2C

Structure	Level	Pattern	Image	Note
TS20: lymphatic system	Present	Regionally restricted	2D	Expression was detected in the jugular lymphatic sacs.
TS20: dermatome	Present	Scattered	2D
TS20: dermis	Present	Scattered	2D

Structure	Level	Pattern	Image	Note
TS19: lymphatic system	Present	Regionally restricted	3A	Expression was detected in the developing jugular lymph sacs.
TS19: cardinal vein	Present	Regionally restricted	3A	Parts of the cardinal vein were stained.
TS19: venous system	Present	Regionally restricted	3A	Segments of the jugular vein were stained.

Structure	Level	Pattern	Image	Note
TS19: dermatome	Present	Scattered	3B
TS19: mediastinum	Present	Scattered	3B

Structure	Level	Pattern	Image	Note
TS17: cardinal vein	Present	Regionally restricted	3C	Expression was detected in specific compartments of the cardinal vein.
TS17: hepatic primordium parenchyma	Present	Regionally restricted	3C	Expression was detected in the hepatic sinusoids.

Structure	Level	Pattern	Image	Note
TS17: vitelline vein	Present		3D
TS17: midgut vascular element	Present	Regionally restricted	3D	Expression was detected in parts of the midgut vascular plexus.