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Gene Expression Data
Assay Details
Assay
Reference: J:119468 Kedees MH, et al., Nestin expression in pancreatic endocrine and exocrine cells of mice lacking glucagon signaling. Dev Dyn. 2007 Mar 15;236(4):1126-1133
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5550102
Gene symbol: Gcg
Gene name: glucagon
Antibody: Anti-Glucagon
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1B,D: embryonic day 11.0 (more )
Note: Double labeled: red - Gcg; green - Nes (assay MGI:5550086).
Structure Level Pattern Image Note
TS18: pancreas primordium Present Clusters 1D , 1B Expression was detected in clusters of cells. Most cells in the clusters co-expressed Glucagon and Nestin.
Specimen not shown: embryonic day 11.0; Gcgrtm1Mjch/Gcgrtm1Mjch (more )
Note: Also stained for Nes (assay MGI:5550086).
Structure Level Pattern Image Note
TS18: pancreas primordium Present Cells co-expressing Nestin and Glucagon were observed in the mutant.
Specimen not shown: embryonic day 11.0; Pcsk2tm1Dfs/Pcsk2tm1Dfs (more )
Note: Also stained for Nes (assay MGI:5550086).
Structure Level Pattern Image Note
TS18: pancreas primordium Present Cells co-expressing Nestin and Glucagon were observed in the mutant.
Specimen 2B: embryonic day 15.0; Gcgrtm1Mjch/Gcgrtm1Mjch (more )
Note: Double labeled: red - Gcg; green - Nes (assay MGI:5550086).
Structure Level Pattern Image Note
TS23: pancreas Present Regionally restricted 2B Co-expression with Nestin was noted in a significant number of cells.
Specimen 3B: postnatal day 1; Pcsk2tm1Dfs/Pcsk2tm1Dfs (more )
Note: Double labeled: red - Gcg; green - Nes (assay MGI:5550086).
Structure Level Pattern Image Note
TS27: pancreas Present 3B The number of cells co-expressing Nestin and Glucagon decreased abruptly and were not observed in the PC2 mutant P1 islets.
Specimen not shown: postnatal day 1; Gcgrtm1Mjch/Gcgrtm1Mjch (more )
Note: Also stained for Nes (assay MGI:5550086).
Structure Level Pattern Image Note
TS27: pancreas Present The number of cells co-expressing Nestin and Glucagon decreased abruptly and were not observed in the Gcgr mutant P1 islets.
Specimen 4B: postnatal month 3; Pcsk2tm1Dfs/Pcsk2tm1Dfs (more )
Structure Level Pattern Image Note
TS28: islets of Langerhans Present 4B
Specimen 4C: postnatal month 3; Pcsk2tm1Dfs/Pcsk2tm1Dfs (more )
Note: Double labeled: red - Gcg; green - Nes (assay MGI:5550086).
Structure Level Pattern Image Note
TS28: islets of Langerhans Present 4C Some cells co-expressed Nestin.
Specimen 4G: postnatal month 3 (more )
Note: Double labeled: red - Gcg; green - Nes (assay MGI:5550086).
Structure Level Pattern Image Note
TS28: islets of Langerhans Present 4G
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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