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Gene Expression Data
Assay Details
Assay
Reference: J:171409 GUDMAP Consortium, GUDMAP: the GenitoUrinary Development Molecular Anatomy Project. www.gudmap.org. 2004;
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5548304
Gene symbol: Nefh
Gene name: neurofilament, heavy polypeptide
Antibody: anti-NF200 (N4142)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Assay notes: Secondary antibody diluted to 1:1000.
Results
Specimen GUDMAP:20939: embryonic day 14.5 (more )
Note: This specimen was generated and annotated by the lab of Janet R. Keast, University of Melbourne.
Structure Level Pattern Image Note
TS22: pelvic ganglion Present GUDMAP:20939
TS22: nerve of bladder Present Graded GUDMAP:20939 lateral expression; Axons associated with surface (serosa/adventitia) and detrusor are primarily in thick bundles in dorsal aspect and thin bundles/single axons in ventral aspect (detrusor only). Axons in all areas are less prevalent than E16-18. Axons are scarce in lamina propria and not seen close to the urothelium.
TS22: nerve of lamina propria of bladder Present GUDMAP:20939 Axons are scarce in lamina propria and not seen close to the urothelium.
TS22: nerve of detrusor muscle of bladder Present Graded GUDMAP:20939 dorsal expression; Axons associated with detrusor are primarily in thick bundles in dorsal aspect and thin bundles/single axons in ventral aspect.
TS22: nerve of outer layer of bladder Present Graded GUDMAP:20939 dorsal expression; Axons associated with surface (serosa/adventitia) are primarily in thick bundles in dorsal aspect.
TS22: nerve of urothelium of bladder Absent GUDMAP:20939
Specimen GUDMAP:20943: embryonic day 18.5 (more )
Note: This specimen was generated and annotated by the lab of Janet R. Keast, University of Melbourne. Double labeled: red = Nefh (NF200); green = either Nos1 (nNOS) or Th (depending on the section)
Structure Level Pattern Image Note
TS26: pelvic ganglion Present GUDMAP:20943 Expression only in axons, not neuronal cell bodies.
TS26: nerve of urethra Present GUDMAP:20943
TS26: nerve of urothelium of bladder Present GUDMAP:20943
TS26: nerve of lamina propria of bladder Present GUDMAP:20943
TS26: nerve of detrusor muscle of bladder Present Graded GUDMAP:20943 dorsal expression; Thicker bundles of axons more prevalent in dorsal tissue; thinner bundles/single axons more prevalent in ventral tissue.
TS26: nerve of outer layer of bladder Present Graded GUDMAP:20943 dorsal expression; Thicker bundles of axons more prevalent in dorsal tissue; thinner bundles/single axons more prevalent in ventral tissue.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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01/06/2026
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