Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:125514
Hartman BH, et al., Dll3 is expressed in developing hair cells in the mammalian cochlea. Dev Dyn. 2007 Oct;236(10):2875-83
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:5547529
|
Gene symbol:
|
Atoh1
|
Gene name:
|
atonal bHLH transcription factor 1
|
Probe:
|
MGC clone 58933
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
Assay notes:
|
Modified Carnoy's fixative: 60% ethanol/30% formaldehyde/10% acetic acid
|
|
Results
|
Specimen
1J,K,L:
embryonic day 15.5
(more )
Specimen
1J,K,L:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Carnoy's Fixative
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: cochlea |
Present |
Non-Uniform |
1J
,
1K
,
1L
|
Strong expression was detected in the presumptive inner hair cells. Weaker, more lateral expression was detected in the presumptive outer hair cell precursors.
|
Specimen
3C,I:
embryonic day 17.0
(more )
Specimen
3C,I:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 17.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Carnoy's Fixative
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: cochlea |
Present |
Regionally restricted |
3C
,
3I
|
Expression level was low in inner hair cells and stronger in outer hair cells in the basal turn.
|
|