Probe:	MGC clone 62324
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Modified Carnoy's fixative: 60% ethanol/30% formaldehyde/10% acetic acid

Structure	Level	Pattern	Image	Note
TS23: cochlea	Present	Regionally restricted	1E , 1D , 1F	Expression was detected in the presumptive inner hair cells in the basal turn (F) and the mid-basal turn (E).
TS23: cochlea epithelium	Present	Regionally restricted	1D , 1E	Expression was observed in columns extending through the thickness of the epithelium.

Structure	Level	Pattern	Image	Note
TS22: cochlea	Strong	Regionally restricted	2D , 2B	Expression was detected in the two basal turns.

Structure	Level	Pattern	Image	Note
TS25: cochlea	Present	Regionally restricted	3B , 3H	Expression level was low in inner hair cells and stronger in outer hair cells in the basal turn.

Structure	Level	Pattern	Image	Note
TS27: cochlea	Present	Regionally restricted	4E , 4B	Levels are strongest in the most apical hair cells, but expression is low or undetectable in hair cells in the more basal regions. Expression is stronger in outer hair cells than inner hair cells. Expression was extremely low in the middle turn.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Regionally restricted		Expression was detected in hair cells only in the most apical turn of the organ of Corti. Expression was absent from all hair cells in the middle and basal turns.

Structure	Level	Pattern	Image	Note
TS28: cochlea	Absent
TS28: retina	Present