Gene Expression Data
Assay Details
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Assay
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Reference:
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J:84414
Haraguchi S, et al., nanos1: a mouse nanos gene expressed in the central nervous system is dispensable for normal development. Mech Dev. 2003 Jun;120(6):721-31
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5545811
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Gene symbol:
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Nanos1
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Gene name:
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nanos C2HC-type zinc finger 1
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
4A:
(close  )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nanos1tm1Ysa/Nanos1tm1Ysa
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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Metaphase II stage oocytes.
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Specimen
4B 8-cell:
embryonic day 2.5; Nanos1tm1Ysa/Nanos1 +
(more  )
Note: 4-cell - blastocyst stage embryos.
Specimen
4B 8-cell:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nanos1tm1Ysa/Nanos1+
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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4-cell - blastocyst stage embryos.
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Specimen
4B blastocyst:
embryonic day 3.5; Nanos1tm1Ysa/Nanos1 +
(more )
Note: 4-cell - blastocyst stage embryos.
Specimen
4B blastocyst:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nanos1tm1Ysa/Nanos1+
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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4-cell - blastocyst stage embryos.
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Specimen
not shown E6.5:
embryonic day 6.5; Nanos1tm1Ysa/Nanos1 +
(more )
Specimen
not shown E6.5:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nanos1tm1Ysa/Nanos1+
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Specimen
not shown E11.5:
embryonic day 11.5; Nanos1tm1Ysa/Nanos1 +
(more )
Specimen
not shown E11.5:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nanos1tm1Ysa/Nanos1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Specimen
4C:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nanos1tm1Ysa/Nanos1tm1Ysa
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Specimen
4D:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nanos1tm1Ysa/Nanos1tm1Ysa
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Specimen
4G,H:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nanos1tm1Ysa/Nanos1tm1Ysa
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Specimen was either +/- or -/-.
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Specimen
4I:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nanos1tm1Ysa/Nanos1tm1Ysa
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Specimen was either +/- or -/-.
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Specimen
4J:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nanos1tm1Ysa/Nanos1tm1Ysa
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Specimen was either +/- or -/-.
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Specimen
4K:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nanos1tm1Ysa/Nanos1tm1Ysa
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Specimen was either +/- or -/-.
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Specimen
5A:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nanos1tm1Ysa/Nanos1tm1Ysa
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Specimen was either +/- or -/-.
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Specimen
5B:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nanos1tm1Ysa/Nanos1tm1Ysa
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Specimen was either +/- or -/-.
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Specimen
5C:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nanos1tm1Ysa/Nanos1tm1Ysa
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Specimen was either +/- or -/-.
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Specimen
5D,E:
postnatal adult; Nanos1tm1Ysa/Nanos1 +
(more )
Specimen
5D,E:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nanos1tm1Ysa/Nanos1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5F:
postnatal adult; Nanos1tm1Ysa/ Nanos1tm1Ysa
(more )
Note: Specimen was either +/- or -/-. Electron microscopy.
Specimen
5F:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nanos1tm1Ysa/Nanos1tm1Ysa
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Specimen was either +/- or -/-. Electron microscopy.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: dentate gyrus |
Present |
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5F
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Expression was detected in both nerve fibers and nerve terminals.
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Specimen
5G:
postnatal adult; Nanos1tm1Ysa/ Nanos1tm1Ysa
(more )
Note: Specimen was either +/- or -/-. Electron microscopy.
Specimen
5G:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nanos1tm1Ysa/Nanos1tm1Ysa
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Specimen was either +/- or -/-. Electron microscopy.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: dentate gyrus |
Present |
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5G
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Expression was detected in both nerve fibers and nerve terminals.
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Specimen
5J:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nanos1tm1Ysa/Nanos1tm1Ysa
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
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5J
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Expression was detected in oocytes at various stages of maturation.
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Specimen
5K:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nanos1tm1Ysa/Nanos1tm1Ysa
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: seminiferous tubule |
Present |
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5K
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Expression was detected in seminiferous tubules at the spermatid stage.
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Specimen
6A:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nanos1tm1Ysa/Nanos1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hippocampus |
Present |
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6A
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Beta-gal staining was higher in the -/- compared to +/-, and the staining pattern differed. Authors indicate the difference between +/- and -/- was due solely to the lacZ allele dosage.
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Specimen
6B:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nanos1tm1Ysa/Nanos1tm1Ysa
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hippocampus |
Present |
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6B
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Beta-gal staining was higher in the -/- compared to +/-, and the staining pattern differed. Authors indicate the difference between +/- and -/- was due solely to the lacZ allele dosage.
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