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Gene Expression Data
Assay Details
Assay
Reference: J:90050 Hart AH, et al., Identification, cloning and expression analysis of the pluripotency promoting Nanog genes in mouse and human. Dev Dyn. 2004 May;230(1):187-98
Assay type: RNA in situ
MGI Accession ID: MGI:5544623
Gene symbol: Nanog
Gene name: Nanog homeobox
Probe: Nanog cDNA3
Probe preparation: Antisense RNA
Assay notes: The authors did not specify which probe was used, this probe or Nanog cDNA4 MGI:5544619, both probes gave similar results.
Results
Specimen 6A: embryonic day 3.0 (more )
Note: Morula stage embryos.
Structure Level Pattern Image Note
TS3: compacted morula Present 6A
Specimen 6B: embryonic day 4.0 (more )
Note: Blastocysts.
Structure Level Pattern Image Note
TS5: inner cell mass Present 6B
Specimen 7A: embryonic day 5.0 (more )
Note: Egg cylinder stage.
Structure Level Pattern Image Note
TS7: epiblast Present Regionally restricted 7A Expression is in the proximal epiblast at the site of the presumptive primitive streak.
Specimen 7B: embryonic day 5.0 (more )
Note: Prestreak stage.
Structure Level Pattern Image Note
TS7: epiblast Strong Regionally restricted 7B Expression is in the region of the proximal epiblast with strongest expression in the region of the streak.
Specimen 7C: embryonic day 6.5 (more )
Note: Early streak stage.
Structure Level Pattern Image Note
TS9: embryo ectoderm Present Regionally restricted 7C Expression is weak in the anterior and strong in posterior epiblast.
Specimen 7D: embryonic day 7.0 (more )
Note: Early bud stage embryo.
Structure Level Pattern Image Note
TS10: embryo ectoderm Present Regionally restricted 7D Expression is weak in the anterior and strong in posterior epiblast.
Specimen 7E: embryonic day 7.25 (more )
Structure Level Pattern Image Note
TS10: embryo ectoderm Present Regionally restricted 7E Expression is weak in the anterior and strong in posterior epiblast.
Specimen 7F: embryonic day 7.5 (more )
Note: Late bud stage embryo.
Structure Level Pattern Image Note
TS11: embryo ectoderm Present Regionally restricted 7F Expression is weak in the anterior and strong in posterior epiblast.
Specimen 7G: embryonic day 6.5 (more )
Structure Level Pattern Image Note
TS9: embryo ectoderm Present Regionally restricted 7G Expression is weak in the anterior and strong in posterior epiblast.
TS9: primitive endoderm Absent 7G
Specimen 7H: embryonic day 7.0 (more )
Structure Level Pattern Image Note
TS10: embryo ectoderm Present Regionally restricted 7H Expression is weak in the anterior and strong in posterior epiblast.
Specimen 7I: embryonic day 7.5 (more )
Structure Level Pattern Image Note
TS11: embryo ectoderm Present Regionally restricted 7I A gradient of expression in epiblast from anterior to posterior, with expression being maximal in epiblast cells flanking the primitive streak. Expression is lost as the cells enter the streak and differentiate to form mesoderm.
Specimen not shown E8: embryonic day 8.0 (more )
Structure Level Pattern Image Note
TS12: embryo Absent
Specimen not shown E11.5: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: gonad Present
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory