Antibody:	anti-mouse versican (GAG-beta domain) (AB1033)
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	Fixative was 2% or 4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS15: atrioventricular canal	Present	Regionally restricted	4B , 4A	Expression is in the extracellular matrix of the endocardial cushions of the atrioventricular canal.
TS15: heart	Present	Regionally restricted	4B , 4A	Expression is in the aortic sac of the anterior heart field and deposits of protein were found spanning acellular spaces between endocardial and myocardial layers.
TS15: endocardial lining	Present		4A
TS15: outflow tract	Present		4A
TS15: primitive ventricle	Present	Regionally restricted	4A	Expression is in the ventricular subendocardium.
TS15: cardiac muscle tissue	Trace		4A	Little or trace was seen in myocardium.

Structure	Level	Pattern	Image	Note
TS17: atrioventricular canal	Strong	Regionally restricted	5A	Expression is in the atrioventricular cushion tissue. Expression is high in the extracellular matrix within the core of the cushion immediately subjacent to the endocardium.

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Strong

Structure	Level	Pattern	Image	Note
TS20: atrioventricular endocardial cushion	Present	Regionally restricted	6B , 6A	Expression is in the extracellular matrix of the mesenchymal core of both inferior and superior cushions and is absent in the extracellular matrix surrounding densely packed cells.

Structure	Level	Pattern	Image	Note
TS22: heart valve	Strong	Regionally restricted	7B , 7A	Expression is in regions of loosely packed mesenchymal cells adjacent to the densely packed cells and is mostly absent in the highly compacted cell region of the atrioventricular valve leaflets, and in the core extending into elongated portion.