Antibody:	anti-Stat3 (C-20) sc-482
Detection system:	Secondary antibody coupled to alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: telencephalon mantle layer	Strong	Regionally restricted	1A	Expressed in a portion of neuroepithelium.
TS17: hindbrain mantle layer	Present		1A
TS17: trigeminal ganglion	Present		1A
TS17: hindbrain	Present		1A	Expressed in the mesenchyme near the mantle layer.

Structure	Level	Pattern	Image	Note
TS20: telencephalon mantle layer	Present	Widespread	1B	Expression extended to the whole mantle layer.
TS20: diencephalon	Present		1B
TS20: medulla oblongata alar plate	Present		1B
TS20: trigeminal ganglion	Present		1B
TS20: pons	Present	Regionally restricted	1B	Expressed in the basal plate.

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Present	Regionally restricted	1C	Expressed in the dorsal region.
TS22: neocortex	Present	Regionally restricted	1C	Expressed in the outer layer.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Present		1D

Structure	Level	Pattern	Image	Note
TS25: cerebellum	Present		1E

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present		1F

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Present		1G

Structure	Level	Pattern	Image	Note
TS17: optic cup outer layer	Present	Regionally restricted	1H	Expressed in the anterior portion of the neuroretina.
TS17: eye mesenchyme	Present	Regionally restricted	1H	Expressed in the mesenchyme near the anterior portion of the neuroretina.
TS17: lens pit	Weak		1H

Structure	Level	Pattern	Image	Note
TS20: lens vesicle	Present	Regionally restricted	1I	Expressed in the equatorial region, where the transition from lens epithelial cells to lens fiber cells takes place.
TS20: optic stalk	Strong		1I

Structure	Level	Pattern	Image	Note
TS22: lens fiber	Strong		1J
TS22: neural retinal epithelium	Strong	Regionally restricted	1J	Expressed in the inner layer.
TS22: optic nerve	Present		1J	Weaker expression.

Structure	Level	Pattern	Image	Note
TS25: lens fiber	Strong		1K	Expressed in primary and secondary lens fibers.
TS25: optic nerve	Strong		1K
TS25: retina inner nuclear layer	Strong		1K

Structure	Level	Pattern	Image	Note
TS27: lens fiber	Present		1L	Expressed in primary and secondary lens fibers.
TS27: neural retina	Present	Regionally restricted	1L	Expressed in the inner nuclear layer of neuroretina.
TS27: optic nerve	Present		1L

Structure	Level	Pattern	Image	Note
TS17: neural tube	Strong	Regionally restricted	2A	Expressed in the mantle zone of the neural tube.
TS17: dorsal root ganglion	Strong		2A

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Strong		2B
TS20: spinal cord mantle layer	Strong		2B
TS20: spinal cord	Strong	Regionally restricted	2B	Expression extended to the central canal but mainly was localized in the mantle layer.

Structure	Level	Pattern	Image	Note
TS22: spinal cord mantle layer	Strong	Non-Uniform	2C	Expression was stronger in the dorsal part. A few strong stained regions in the ventral most part, which may correspond to the location of motoneuron columns.
TS22: spinal cord	Present	Non-Uniform	2C	Expression was stronger in the dorsal part. A few strong stained regions in the ventral most part, which may correspond to the location of motoneuron columns. No expression in the peripheral layer.

Structure	Level	Pattern	Image	Note
TS25: spinal cord	Present	Uniform	2D	Expressed uniformly throughout the neural tube, and there was still some strong staining in the ventral most part of the mantle layer.
TS25: spinal cord mantle layer	Strong	Regionally restricted	2D	Expressed in the ventral most part.

Structure	Level	Pattern	Image	Note
TS27: spinal cord ventral horn	Present		2E
TS27: spinal cord	Weak	Regionally restricted	2E	Expression was scattered and very weak, except in the region of the ventral horns.