Probe:	Tgfb3 probe5
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: heart	Absent

Structure	Level	Pattern	Image	Note
TS17: heart	Absent

Structure	Level	Pattern	Image	Note
TS19: blood vessel	Present	Regionally restricted		Expression is in the smooth muscle cells of the major blood vessels.
TS19: visceral pericardium	Present			Expression is in the epicardium.
TS19: endocardial cushion	Absent

Structure	Level	Pattern	Image	Note
TS20: arterial system	Present	Regionally restricted	3D	Expression is throughout the media and adventitia (smooth muscle cells, fibroblasts) of the great arteries.
TS20: outflow tract	Present	Regionally restricted	3D	Expression is in the endocardial cushions of the outflow tract.
TS20: ascending aorta	Present		3D
TS20: atrioventricular endocardial cushion	Present		3D
TS20: endocardial cushion	Present		3D
TS20: visceral pericardium	Present		3D
TS20: heart ventricle	Present	Regionally restricted	3D	Expression is in the epicardium lining the ventricles with a higher signal in the more densely populated atrio-ventricular sulcus.
TS20: heart	Weak	Regionally restricted	3D	Expression is in the epicardium covering the atria, the myocardium of the atrioventricular sulcus, and ventricles.

Structure	Level	Pattern	Image	Note
TS20: arterial system	Present	Regionally restricted	3H	Expression is throughout the media and adventitia (smooth muscle cells, fibroblasts) of the great arteries.
TS20: outflow tract	Present	Regionally restricted	3H	Expression is in the endocardial cushions of the outflow tract.
TS20: ascending aorta	Present		3H
TS20: atrioventricular endocardial cushion	Present		3H

Structure	Level	Pattern	Image	Note
TS20: heart ventricle	Present	Regionally restricted	3L	Expression is in the epicardium lining the ventricles with a higher signal in the more densely populated atrio-ventricular sulcus.
TS20: visceral pericardium	Present	Regionally restricted	3L	Expression is in the epicardium covering the atria, the myocardium of the atrioventricular sulcus, and ventricles.

Structure	Level	Pattern	Image	Note
TS20: visceral pericardium	Present	Regionally restricted	3P	Expression is in the epicardium covering the atria, the myocardium of the atrioventricular sulcus, and ventricles.

Structure	Level	Pattern	Image	Note
TS21: ascending aorta	Present		4D
TS21: endocardial cushion	Present		4D

Structure	Level	Pattern	Image	Note
TS21: ascending aorta	Present		4H
TS21: outflow tract	Present	Regionally restricted	4H	Expression is in the cushions of the outflow tract.

Structure	Level	Pattern	Image	Note
TS21: ascending aorta	Present		4L
TS21: outflow tract	Present	Regionally restricted	4L	Expression is in the cushions of the outflow tract.

Structure	Level	Pattern	Image	Note
TS22: heart valve	Present	Regionally restricted	5D

Structure	Level	Pattern	Image	Note
TS22: heart valve	Present	Regionally restricted	5H	Expression is in the differentiating semilunar valve leaflets with a higher level in the valve annuli and the valvular ring.
TS22: arterial system	Present		5H
TS22: outflow tract	Present	Regionally restricted	5H	Expression intensity in the septum decreases and remains confined to its residual mesenchymal area directly below the aortic semilunar valve.

Structure	Level	Pattern	Image	Note
TS23: atrium endocardial lining	Present		6D
TS23: cardiac valve leaflet	Present		6D
TS23: visceral pericardium	Present		6D
TS23: interatrial septum	Present	Regionally restricted	6D	Expression is in the atrial septum except for its myocardial base that connects with the atrio-ventricular valves.
TS23: heart ventricle	Present	Regionally restricted	6D	Expression is in the lining.

Structure	Level	Pattern	Image	Note
TS23: arterial system	Present		6H
TS23: atrium endocardial lining	Present		6H
TS23: visceral pericardium	Present		6H
TS23: cardiac valve leaflet	Present	Regionally restricted	6H	Expression is in the differentiating semilunar valve leaflets with a higher level in the valve annuli and the valvular ring.
TS23: heart ventricle	Present	Regionally restricted	6H	Expression is in the lining.

Structure	Level	Pattern	Image	Note
TS23: arterial system	Present		6L
TS23: atrium endocardial lining	Present		6L
TS23: heart ventricle	Present	Regionally restricted	6L	Expression is in the lining.

Structure	Level	Pattern	Image	Note
TS23: atrium endocardial lining	Present		6P
TS23: visceral pericardium	Present		6P
TS23: heart ventricle	Present	Regionally restricted	6P	Expression is in the lining.

Structure	Level	Pattern	Image	Note
TS23: atrium endocardial lining	Present		6T
TS23: visceral pericardium	Present		6T
TS23: heart ventricle	Present	Regionally restricted	6T	Expression is in the lining.