Probe:	Tgfb1 probe7
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Strong		1B
TS15: dorsal mesocardium	Strong		1B
TS15: endocardial lining	Strong		1B
TS15: branchial arch artery	Present		1B
TS15: mesenchyme derived from splanchnopleure	Present		1B
TS15: venous system	Present	Regionally restricted	1B	Expression is in the endothelium of the systemic veins.
TS15: arterial system	Present	Regionally restricted	1B	Expression is in the endothelium of the aortic sac.
TS15: cardiac muscle tissue	Absent		1B

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Strong		1E
TS15: dorsal mesocardium	Strong		1E
TS15: endocardial lining	Strong		1E	Stronger signal in the more densily populated endocardial lining of the cushions on the atrioventricular canal and covering the ventricular trabeculae.
TS15: cardiac muscle tissue	Absent		1E

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Strong		1H
TS15: dorsal mesocardium	Strong		1H
TS15: endocardial lining	Strong		1H
TS15: venous system	Present	Regionally restricted	1H	Expression is in the endothelium of the systemic veins.
TS15: arterial system	Present	Regionally restricted	1H	Expression is in the endothelium of the aortic sac.
TS15: branchial arch artery	Present		1H
TS15: cardiac muscle tissue	Absent		1H

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present
TS17: dorsal mesocardium	Present
TS17: endocardial lining	Present
TS17: cardiac muscle tissue	Absent

Structure	Level	Pattern	Image	Note
TS19: endocardial lining	Present	Regionally restricted	2B	Expression is in the endocardium overlying the antrio-ventricular canal, the outflow tract, and the myocardial trabeculations.
TS19: blood vessel	Present	Regionally restricted	2B	Expression is in the endothelium.
TS19: endocardial cushion	Weak		2B
TS19: atrioventricular canal	Absent		2B
TS19: dorsal mesocardium	Absent		2B

Structure	Level	Pattern	Image	Note
TS19: blood vessel	Present	Regionally restricted	2E	Expression is in the endothelium.
TS19: endocardial lining	Present	Regionally restricted	2E	Expression is in the endocardium overlying the antrio-ventricular canal, the outflow tract, and the myocardial trabeculations.
TS19: endocardial cushion	Weak		2E
TS19: dorsal mesocardium	Absent		2E

Structure	Level	Pattern	Image	Note
TS19: endocardial lining	Present		2H

Structure	Level	Pattern	Image	Note
TS19: endocardial lining	Present		2K

Structure	Level	Pattern	Image	Note
TS20: endocardial lining	Present		3B	Expression is strongest overlying the cushions.
TS20: visceral pericardium	Present	Regionally restricted	3B	Scattered expression in the epicardium of the atrioventricular sulcus and is almost absent in the lining of the apex.
TS20: heart atrium	Trace		3B

Structure	Level	Pattern	Image	Note
TS20: endocardial lining	Present		3F	Expression is strongest overlying the cushions.

Structure	Level	Pattern	Image	Note
TS20: visceral pericardium	Present	Regionally restricted	3J	Scattered expression in the epicardium of the atrioventricular sulcus and is almost absent in the lining of the apex.
TS20: heart atrium	Trace		3J

Structure	Level	Pattern	Image	Note
TS20: visceral pericardium	Present	Regionally restricted	3N	Scattered expression in the epicardium of the atrioventricular sulcus and is almost absent in the lining of the apex.

Structure	Level	Pattern	Image	Note
TS21: heart valve	Present	Regionally restricted	4B	Expression is in the endocardium.
TS21: blood vessel	Present	Regionally restricted	4B	Expression is in the endothelium.

Structure	Level	Pattern	Image	Note
TS21: heart valve	Present	Regionally restricted	4F	Expression is in the endocardium.
TS21: blood vessel	Present	Regionally restricted	4F	Expression is in the endothelium.
TS21: visceral pericardium	Present	Regionally restricted	4F	Expression is in foci in the epicardium in the proximity of the outflow tract.

Structure	Level	Pattern	Image	Note
TS21: heart valve	Present	Regionally restricted	4J	Expression is in the endocardium.
TS21: blood vessel	Present	Regionally restricted	4J	Expression is in the endothelium.

Structure	Level	Pattern	Image	Note
TS22: heart valve	Present	Regionally restricted	5B	Expression is in the endocardium.
TS22: blood vessel	Present	Regionally restricted	5B	Expression is in the endothelium.

Structure	Level	Pattern	Image	Note
TS22: heart valve	Present	Regionally restricted	5F	Expression is in the endocardium.
TS22: blood vessel	Present	Regionally restricted	5F	Expression is in the endothelium.

Structure	Level	Pattern	Image	Note
TS23: heart valve	Present	Regionally restricted	6B	Expression is in the endocardium.
TS23: blood vessel	Present	Regionally restricted	6B	Expression is in the endothelium.

Structure	Level	Pattern	Image	Note
TS23: heart valve	Present	Regionally restricted	6F	Expression is in the endocardium.
TS23: blood vessel	Present	Regionally restricted	6F	Expression is in the endothelium.
TS23: visceral pericardium	Present	Regionally restricted	6F	Expression is in foci in the epicardium in the proximity of the outflow tract.

Structure	Level	Pattern	Image	Note
TS23: heart valve	Present	Regionally restricted	6J	Expression is in the endocardium.
TS23: blood vessel	Present	Regionally restricted	6J	Expression is in the endothelium.

Structure	Level	Pattern	Image	Note
TS23: heart	Present	Regionally restricted	6N

Structure	Level	Pattern	Image	Note
TS23: heart ventricle	Present	Regionally restricted	6R	Expression is in a few foci of the epicardium of the ventricles.