Probe:	Ehox cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS9: extraembryonic ectoderm	Present	Regionally restricted	2A	Expressed in the extraembryonic ectoderm with the most intense staining in the cells adjacent to the epiblast, where the rapidly dividing trophoblast stem cells locate.

Structure	Level	Pattern	Image	Note
TS11: chorion	Present		2B

Structure	Level	Pattern	Image	Note
TS12: chorion	Strong		2C

Structure	Level	Pattern	Image	Note
TS12: chorion	Strong	Regionally restricted	2D	Expressed in the chorionic membrane surrounding the ectoplacental cavity, which will later become part of the chorioallantoic placenta.
TS12: ectoplacental cone	Weak	Regionally restricted	2D	Expressed in some cells.

Structure	Level	Pattern	Image	Note
TS23: labyrinthine zone	Present		3_left
TS23: spongiotrophoblast	Present		3_left
TS23: trophoblast giant cell	Present	Regionally restricted	3_left	Expressed in giant cells at the edge of the placenta.

Structure	Level	Pattern	Image	Note
TS23: labyrinthine zone	Present		3_right
TS23: spongiotrophoblast	Present		3_right
TS23: trophoblast giant cell	Present	Regionally restricted	3_right	Expressed in giant cells at the edge of the placenta.

Structure	Level	Pattern	Image	Note
TS13: thyroid primordium	Present		4B , 4A
TS13: foregut diverticulum	Present	Regionally restricted	4A , 4B	Expressed in two distinct regions on the ventral side of the anterior gut tube.

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present	Regionally restricted	4C	Expressed in the pharyngeal endoderm surrounding the thyroid primordium.

Structure	Level	Pattern	Image	Note
TS16: foregut	Present	Regionally restricted	5A	Expressed in the ventral floor of the foregut from the second to the fourth pharyngeal pouches.
TS16: 1st branchial pouch	Present		5A
TS16: 2nd branchial pouch	Present		5A
TS16: 3rd branchial pouch	Present		5A
TS16: 4th branchial pouch	Present		5A

Structure	Level	Pattern	Image	Note
TS16: 1st branchial pouch	Present		5B
TS16: 2nd branchial pouch	Present		5B
TS16: 3rd branchial pouch	Present		5B
TS16: 4th branchial pouch	Present		5B

Structure	Level	Pattern	Image	Note
TS16: 1st branchial pouch	Present		5C
TS16: 2nd branchial pouch	Present		5C
TS16: 3rd branchial pouch	Present		5C
TS16: 4th branchial pouch	Present		5C

Structure	Level	Pattern	Image	Note
TS17: 3rd branchial pouch	Present	Regionally restricted	6A	Expressed in the ventral end.
TS17: 2nd branchial pouch	Weak	Regionally restricted	6A	Some expression remianed in the ventral end.

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Absent