Antibody:	anti-alpha6 integrin (GoH3)
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	The fixative was 0.2% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS19: somite	Present	Regionally restricted	2A	Expression was detected in epithelial somites.
TS19: dermomyotome	Present		2A
TS19: myotome	Present		2A
TS19: tail unsegmented mesenchyme	Absent		2A
TS19: paraxial mesenchyme	Present	Regionally restricted	2A	Expression was concentrated on the basal surface of the cells forming the cleft separating the first epithelial somite from the presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS19: somite	Present	Regionally restricted	2C	Expression was present on the surface of cells in epithelial somites.

Structure	Level	Pattern	Image	Note
TS14: myotome	Present		2D	Expression was stronger in the myotome than in the dermomyotome. All Myf-5 positive cells in the myotome were also alpha-6 positive.
TS14: dermomyotome	Present		2D	Expression was present in the whole dermomyotome. All Myf5-positive cells in the dorsomedial lip of the dermomyotome also express alpha-6.

Structure	Level	Pattern	Image	Note
TS19: dermomyotome	Strong		2E	Expression increased in the dermomyotome compared to the 15-somite stage.

Structure	Level	Pattern	Image	Note
TS15: myotome	Present		2F	Expression was detected on Myogenin-positive cells in the myotome.

Structure	Level	Pattern	Image	Note
TS19: trunk myotome	Present		2K

Structure	Level	Pattern	Image	Note
TS19: myotome	Weak	Regionally restricted	2L	Expression was weaker, almost absent, in rostral myotomes.

Structure	Level	Pattern	Image	Note
TS19: myotome	Present		3A	There was no overlap with MyoD-expressing cells in young epaxial cells near the dorsomedial lip.

Structure	Level	Pattern	Image	Note
TS19: myotome	Present		3B	There was no overlap with MyoD-expressing cells in rostral mature myotomes. The hypaxial domain was negative for expression.

Structure	Level	Pattern	Image	Note
TS19: trunk myotome	Present		3C	Some MyoD-positive nuclei are detected in trunk myotomes in areas containing alpha-6-positive elongated myocytes.

Structure	Level	Pattern	Image	Note
TS19: myotome	Present	Regionally restricted	3D , 3F	At the interlimb level of the trunk, there was strong expression in the intercalated myotome but faint expression in the ventrolateral (hypaxial) portion of the myotome compared to Myogenin staining the whole myotome.