Probe:	Prx1 cDNA
Probe preparation:	Antisense labelled with radioactivity RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS28: mesometrium	Present	Regionally restricted	2A	Expressed in mesenchymal cells directly underlying the remnants of the epithelium.
TS28: decidua	Present	Regionally restricted	2A	Expressed in the periphery of the deciduum in a subset cells.

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	2C	Expression is absent from the primitive streak and its surroundings. Expressed in most of the ectoderm, including the precursors of the brain.
TS11: embryo mesoderm	Present	Regionally restricted	2C	Expression is absent from the primitive streak and its surroundings. Expressed in most of the ectoderm, including the precursors of the brain.
TS11: allantois	Present	Regionally restricted	2C	Expressed in a part of the allantois.
TS11: primitive streak	Absent		2C
TS11: future brain	Present		2C
TS11: heart	Present		2C

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	2D	Expression is absent from the primitive streak and its surroundings. Expressed in most of the ectoderm, including the precursors of the brain.
TS11: embryo mesoderm	Present	Regionally restricted	2D	Expression is absent from the primitive streak and its surroundings. Expressed in most of the ectoderm, including the precursors of the brain.
TS11: allantois	Present	Regionally restricted	2D	Expressed in a part of the allantois.
TS11: future brain	Present		2D

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present		3A left/middle
TS15: dermomyotome	Present		3A left/middle
TS15: mesenchyme derived from somatopleure	Present		3A left/middle
TS15: surface ectoderm	Present	Regionally restricted	3A left/middle	Expressed in the dermis.

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Present	Regionally restricted	3B	Expressed in a broader part of the cranial mesenchyme.
TS17: branchial arch	Present		3B
TS17: dermomyotome	Present		3B
TS17: surface ectoderm	Present	Regionally restricted	3B	Expressed in the dermis.
TS17: body wall	Present	Regionally restricted	3B	Expressed in the somatopleure.
TS17: genitourinary system mesenchyme	Present	Regionally restricted	3B	Expressed in mesenchyme surrounding the mesonephric duct.

Structure	Level	Pattern	Image	Note
TS13: heart	Present	Regionally restricted	5A/B	Expressed in the inner curvature in the atrioventricular region.
TS13: dorsal mesocardium	Present	Regionally restricted	5A/B	Expressed in the mesenchyme.
TS13: cardiac muscle tissue	Absent		5A/B	No expression in the myocardium of the developing atria and ventricles.
TS13: endocardial tube	Absent		5A/B	No expression in the heart endocardial lining.

Structure	Level	Pattern	Image	Note
TS13: splanchnopleure	Present		5C/D	Expressed in the splanchnic mesoderm.
TS13: heart	Present	Regionally restricted	5C/D	Expressed in the inner curvature in the atrioventricular region.
TS13: dorsal mesocardium	Present	Regionally restricted	5C/D	Expressed in the mesenchyme.
TS13: cardiac muscle tissue	Absent		5C/D	No expression in the myocardium of the developing atria and ventricles.
TS13: endocardial tube	Absent		5C/D	No expression in the heart endocardial lining.

Structure	Level	Pattern	Image	Note
TS20: atrioventricular endocardial cushion	Strong		5E/F
TS20: outflow tract	Present	Regionally restricted	5E/F	High expression in the outflow tract ridge, but absence of signal in the aortic wall.
TS20: endocardial lining	Strong	Regionally restricted	5E/F	High expression in the overlying endocardium of the atrioventricular and outflow tract cushions.
TS20: aortico-pulmonary spiral septum	Present	Regionally restricted	5E/F	Expressed in the condensed mesenchyme of the aorto-pulmonary septum.
TS20: ascending aorta	Weak		5E/F
TS20: pulmonary artery	Weak		5E/F
TS20: cardinal vein	Present	Regionally restricted	5E/F	Expressed in the smooth-muscle cells of the walls of the tunica media as well as in the lining endothelium.
TS20: dorsal aorta	Present	Regionally restricted	5E/F	Expressed in the smooth-muscle cells of the walls of the tunica media as well as in the lining endothelium.

Structure	Level	Pattern	Image	Note
TS22: heart valve	Present		6A/D	Expressed in the developing semilunar and atrioventricular valves.
TS22: ascending aorta	Present		6A/D	Expressed in a lower level.
TS22: pulmonary trunk	Present		6A/D	Expressed in a lower level.
TS22: dorsal aorta	Present		6A/D	Expressed in a higher level.
TS22: ductus arteriosus	Present		6A/D	Expressed in a higher level.
TS22: superior vena cava	Present		6A/D	Expressed in the cardinal veins.

Structure	Level	Pattern	Image	Note
TS22: heart valve	Present		6B/E	Expressed in the developing semilunar and atrioventricular valves.
TS22: superior vena cava	Present		6B/E	Expressed in the cardinal veins.
TS22: dorsal aorta	Present		6B/E
TS22: ductus arteriosus	Present		6B/E

Structure	Level	Pattern	Image	Note
TS22: heart valve	Present		6C/F	Expressed in the developing semilunar and atrioventricular valves.
TS22: body wall mesenchyme	Present	Regionally restricted	6C/F	Expressed in the splanchnic mesoderm at the level of the venous pole of the heart.
TS22: superior vena cava	Present		6C/F	Expressed in the cardinal veins.
TS22: dorsal aorta	Present		6C/F
TS22: ductus arteriosus	Present		6C/F
TS22: heart	Present	Regionally restricted	6C/F	Expressed at the venous pole of the heart the sinus venosus area, including the pulmonary veins and the connection of the dorsal mesocardium through the spina vestibuli.

Structure	Level	Pattern	Image	Note
TS25: heart valve	Present		7A	Expressed in the semilunar and atrioventricular valve areas.
TS25: heart mesentery	Present	Regionally restricted	7A	Expressed in the future fibrous heart skeleton including the fibrous trigone.

Structure	Level	Pattern	Image	Note
TS25: heart valve	Present		7B	Expressed in the semilunar and atrioventricular valve areas.
TS25: heart mesentery	Present	Regionally restricted	7B	Expressed in the future fibrous heart skeleton including the fibrous trigone.

Structure	Level	Pattern	Image	Note
TS25: heart valve	Present		7C	Expressed in the semilunar and atrioventricular valve areas.
TS25: heart mesentery	Present	Regionally restricted	7C	Expressed in the future fibrous heart skeleton including the fibrous trigone.

Structure	Level	Pattern	Image	Note
TS25: humerus	Absent		9A
TS25: ulna	Absent		9A
TS25: forelimb mesenchyme	Present	Regionally restricted	9A	Expressed in a thin layer of one or two cells around the chondrogenic centers, in or near the perichondrium. Occasionally expressed in a thin layer skirting the muscle, which may correspond to expression in the presumptive fascia.
TS25: forelimb skin	Present	Regionally restricted	9A	Expressed in the dermis.

Structure	Level	Pattern	Image	Note
TS25: palmar pad	Present		9B
TS25: hand mesenchyme	Present	Regionally restricted	9B	Expressed in tendons and near perichondrium.
TS25: forelimb digit 3 metacarpal	Absent		9B
TS25: forelimb digit 4 metacarpal	Absent		9B

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	10C , 10A/B	Expressed in an initially small and distinct region, located in the mammillary area.
TS15: telencephalon	Present	Regionally restricted	10A/B , 10C	Expressed in a rather broad domain.
TS15: branchial arch	Strong		10A/B , 10C
TS15: forelimb bud	Strong		10A/B , 10C
TS15: hindlimb ridge	Strong		10A/B , 10C
TS15: head mesenchyme	Present		10C , 10A/B

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	10D/E	No expression in an area corresponding to a few cells in the midline. Expressed in an initially small and distinct region, located in the mammillary area.
TS17: telencephalon	Present	Regionally restricted	10D/E	Expressed in a more restricted pattern.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	10F/G	Expressed in an initially small and distinct region, located in the mammillary area.
TS17: telencephalon	Present	Regionally restricted	10F/G	Expressed in a more restricted pattern.
TS17: forebrain	Present	Regionally restricted	10F/G	Expressed as a butterfly-like domain with expression extending laterally more rostrally.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	10I/J	Expressed in a more restricted pattern.
TS20: diencephalon	Present	Regionally restricted	10I/J	Expressed in the ependymal layer of the mammillary area.

Structure	Level	Pattern	Image	Note
TS20: spinal cord lateral wall	Present	Regionally restricted	10L/M	Expressed more laterally, not detectable in the midline area.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	10O/P	Expressed in an area surrounding the entrance point of the optic nerve.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	10Q/R	Expressed in an area surrounding the entrance point of the optic nerve.

Structure	Level	Pattern	Image	Note
TS27: 3rd ventricle	Present	Regionally restricted	10S/T	Expressed antero-ventrally from the third ventricle in an area extending from the infundibular recess to the preoptic recess. High expression in the ventral ventricular zone.
TS27: hypothalamic nucleus	Present	Regionally restricted	10S/T	Expressed in the ventromedial hypothalamic nucleus.
TS27: mammillary body	Absent		10S/T
TS27: brain meninges	Strong		10S/T

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted		Expressed in the periphery of the deciduum in a subset cells. Longer exposures demonstrated expression in the outer layers.
TS28: mesometrium	Present	Regionally restricted		Expressed in mesenchymal cells directly underlying the remnants of the epithelium.

Structure	Level	Pattern	Image	Note
TS13: 1st branchial arch	Present
TS13: yolk sac mesenchyme	Present
TS28: decidua	Present	Regionally restricted		Expressed in the periphery of the deciduum in a subset cells. Longer exposures demonstrated expression in the outer layers.
TS13: branchial arch	Strong
TS13: splanchnopleure	Strong	Regionally restricted		Expressed in the splanchnic mesoderm surrounding the gut.

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Strong
TS15: mesenchyme derived from somatopleure	Strong	Regionally restricted		Expressed in the splanchnic mesoderm surrounding the gut.
TS15: cardiac muscle tissue	Absent			No expression in the myocardium of the developing atria and ventricles.
TS15: endocardial lining	Absent