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Gene Expression Data
Assay Details
Assay
Reference: J:151374 Teichert AM, et al., Endothelial nitric oxide synthase gene expression during murine embryogenesis: commencement of expression in the embryo occurs with the establishment of a unidirectional circulatory system. Circ Res. 2008 Jul 3;103(1):24-33
Assay type: RNA in situ
MGI Accession ID: MGI:5524970
Gene symbol: Nos3
Gene name: nitric oxide synthase 3, endothelial cell
Probe: eNOS probe
Probe preparation: Antisense labelled with S35 RNA
Visualized with: Autoradiography
Assay notes: Probess were alkali hydrolyzed to a mean size of 70 nucleotides.
Results
Specimen 6A: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: blood vessel Present Regionally restricted 6A Expression is in the endothelium of developing blood vessels, including the arteries of the aortic arch, intersomitic arteries, vessels of the limb bud, and vessels at the periphery of the neural tube and the brain.
TS15: endocardial lining Present 6A

Specimen 6B: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: blood vessel Present Regionally restricted 6B Expression is in the larger blood vessels of the developing pharynx, in the central vessels of the limb bud, and is strong in the intersomitic arteries.
TS19: atrium endocardial lining Present 6B
TS19: basilar artery Present 6B
TS19: dorsal aorta Present 6B
TS19: umbilical artery Present 6B
TS19: umbilical vein Present 6B
TS19: ventricle endocardial lining Present 6B

Specimen 6C: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: blood vessel Present Regionally restricted 6C Expression is restricted to medium- to large-sized blood vessels and include vessels of the hindlimb, vessels of the stomach, intestine, lung, and in endothelial cells of the blood vessels surrounding the forebrain and hindbrain.

Specimen 6D: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: blood vessel Present Regionally restricted 6D Expression is restricted to medium- to large-sized blood vessels and include vessels of the hindlimb, vessels of the stomach, intestine, lung, and in endothelial cells of the blood vessels surrounding the forebrain and hindbrain.

Specimen not shown E17.5: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: atrium endocardial lining Present
TS25: ventricle endocardial lining Present

Specimen not shown E11.5: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: apical ectodermal ridge Present


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory