Gene Expression Data
Assay Details
Assay
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Reference:
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J:56939
Miquerol L, et al., Multiple developmental roles of VEGF suggested by a LacZ-tagged allele. Dev Biol. 1999 Aug 15;212(2):307-22
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5512645
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Gene symbol:
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Kdr
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Gene name:
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kinase insert domain protein receptor
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Fixative was 0.2% glutaraldehyde, 2% paraformaldehyde for embryos <E10.5. Later stage embryos (>E10.5) were fixed 4% paraformaldehyde, embedded in Tissue Tek (OCT compound), frozen and cryosectioned.
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Results
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Specimen
3A:
embryonic day 8.0; Kdrtm1Jrt/Kdr +
(more )
Specimen
3A:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Kdrtm1Jrt/Kdr+
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: blood vessel |
Present |
Regionally restricted |
3A
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Expression is in endothelial cells.
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Specimen
3B:
embryonic day 8.5; Kdrtm1Jrt/Kdr +
(more )
Specimen
3B:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Kdrtm1Jrt/Kdr+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: blood vessel |
Present |
Regionally restricted |
3B
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Expression is in endothelial cells.
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Specimen
3C:
embryonic day 9.0; Kdrtm1Jrt/Kdr +
(more )
Specimen
3C:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Kdrtm1Jrt/Kdr+
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: blood vessel |
Present |
Regionally restricted |
3C
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Expression is in endothelial cells.
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Specimen
4L:
embryonic day 13.5; Kdrtm1Jrt/Kdr +
(more )
Specimen
4L:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Kdrtm1Jrt/Kdr+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
4M:
embryonic day 13.5; Kdrtm1Jrt/Kdr +
(more )
Specimen
4M:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Kdrtm1Jrt/Kdr+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
5E:
embryonic day 13.5; Kdrtm1Jrt/Kdr +
(more )
Specimen
5E:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Kdrtm1Jrt/Kdr+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS21: blood vessel |
Strong |
Regionally restricted |
5E
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Expression is in vessels surrounding the ureteric bud and not at the periphery of the kidney.
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TS21: metanephros |
Strong |
Regionally restricted |
5E
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Expression is in vessels surrounding the ureteric bud and not at the periphery of the kidney.
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Specimen
5I:
embryonic day 13.5; Kdrtm1Jrt/Kdr +
(more )
Specimen
5I:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
|
Kdrtm1Jrt/Kdr+
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Age:
|
embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS21: blood vessel |
Present |
Regionally restricted |
5I
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Expression is in the vasculature.
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TS21: lung |
Present |
Regionally restricted |
5I
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Expression is in the vasculature.
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Specimen
5K:
embryonic day 13.5; Kdrtm1Jrt/Kdr +
(more )
Specimen
5K:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Kdrtm1Jrt/Kdr+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS22: blood vessel |
Present |
Regionally restricted |
5K
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Expression is in the vasculature of the spleen.
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Specimen
6E:
embryonic day 13.5; Kdrtm1Jrt/Kdr +
(more )
Specimen
6E:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Kdrtm1Jrt/Kdr+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS21: adrenal gland |
Present |
Regionally restricted |
6E
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Expression is in endothelial cells.
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TS21: blood vessel |
Present |
Regionally restricted |
6E
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Expression is in endothelial cells.
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Specimen
6F:
embryonic day 13.5; Kdrtm1Jrt/Kdr +
(more )
Specimen
6F:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Kdrtm1Jrt/Kdr+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: blood vessel |
Present |
Regionally restricted |
6F
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Expression is in endothelial cells.
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TS21: choroid plexus |
Present |
Regionally restricted |
6F
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Expression is in endothelial cells.
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Specimen
6G:
embryonic day 13.5; Kdrtm1Jrt/Kdr +
(more )
Specimen
6G:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Kdrtm1Jrt/Kdr+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
6H:
embryonic day 13.5; Kdrtm1Jrt/Kdr +
(more )
Specimen
6H:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Kdrtm1Jrt/Kdr+
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Age:
|
embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS21: blood vessel |
Present |
Regionally restricted |
6H
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Expression is in endothelial cells.
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TS21: stomach |
Present |
Regionally restricted |
6H
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Expression is in endothelial cells.
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