Gene Expression Data
Assay Details
Assay
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Reference:
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J:187373
Plachez C, et al., Expression of nuclear factor one A and -B in the olfactory bulb. J Comp Neurol. 2012 Oct 1;520(14):3135-49
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5510846
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Gene symbol:
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Nfia
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Gene name:
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nuclear factor I/A
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Antibody:
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Anti-NFIA
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Detection system:
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Secondary antibody coupled to Alexa Fluor 594
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Results
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Specimen
3C/D:
postnatal adult; Gad1tm1.1Tama/Gad1 +
(more )
Note: Triple labeled: magenta - Nfia; green - Gad1 reporter (assay MGI:5510847); blue - DAPI.
Specimen
3C/D:
(close )
Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Gad1tm1.1Tama/Gad1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: magenta - Nfia; green - Gad1 reporter (assay MGI:5510847); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: main olfactory bulb |
Present |
|
3D
,
3C
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Expression in the olfactory bulb did not coincide with GAD67-GFP expression.
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Specimen
3G/H:
postnatal adult
(more )
Note: Triple labeled: magenta - Nfia; green - Tubb3 (assay MGI:5510853); blue - DAPI.
Specimen
3G/H:
(close )
Genetic Background:
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C57BL/6J
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Note:
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Triple labeled: magenta - Nfia; green - Tubb3 (assay MGI:5510853); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: main olfactory bulb |
Present |
Regionally restricted |
3H
,
3G
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Expression did not coincide with TuJ1-positive mitral cells in the olfactory bulb.
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Specimen
3K/L:
postnatal adult
(more )
Note: Triple labeled: magenta - Nfia; green - Gfap (assay MGI:5510854); blue - DAPI.
Specimen
3K/L:
(close )
Genetic Background:
|
C57BL/6J
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Age:
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postnatal adult
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: magenta - Nfia; green - Gfap (assay MGI:5510854); blue - DAPI.
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Specimen
7B:
postnatal adult
(more )
Note: Double labeled: magenta - Nfia; green - Gfap (assay MGI:5510854).
Specimen
7B:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: magenta - Nfia; green - Gfap (assay MGI:5510854).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex subventricular zone |
Present |
Regionally restricted |
7B
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Expression was detected in cells lining the lateral ventricles. Some immunopositive cells were surrounded by GFAP-positive fibers, indicating that these may be neural progenitor cells.
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Specimen
7C:
postnatal adult
(more )
Note: Double labeled: magenta - Nfia; green - Gfap (assay MGI:5510854).
Specimen
7C:
(close )
Genetic Background:
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C57BL/6J
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: magenta - Nfia; green - Gfap (assay MGI:5510854).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex subventricular zone |
Present |
Regionally restricted |
7C
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Expression was detected in cells lining the lateral ventricles. Some immunopositive cells were surrounded by GFAP-positive fibers, indicating that these may be neural progenitor cells.
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Specimen
8C/D:
postnatal adult; Gad1tm1.1Tama/Gad1 +
(more )
Note: Triple labeled: magenta - Nfia; green - Gad1 reporter (assay MGI:5510847); blue - DAPI.
Specimen
8C/D:
(close )
Genetic Background:
|
involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
|
Gad1tm1.1Tama/Gad1+
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Triple labeled: magenta - Nfia; green - Gad1 reporter (assay MGI:5510847); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: rostral migratory stream |
Present |
Regionally restricted |
8C
,
8D
|
Expression was detected in cells of the rostral migratory stream. Some cells were also positive for GAD67-GFP, however some cells were GFP-negative.
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Specimen
8K/L:
postnatal adult
(more )
Note: Triple labeled: magenta - Nfia; green - Gfap (assay MGI:5510854); blue - DAPI.
Specimen
8K/L:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Triple labeled: magenta - Nfia; green - Gfap (assay MGI:5510854); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: rostral migratory stream |
Present |
Regionally restricted |
8L
,
8K
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Expression was detected in cells of the rostral migratory stream. Some immunopositive cells were also GFAP-expressing astrocytes.
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