Gene Expression Data
Assay Details
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Assay
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Reference:
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J:187356
Aroca P, et al., Locus coeruleus neurons originate in alar rhombomere 1 and migrate into the basal plate: Studies in chick and mouse embryos. J Comp Neurol. 2006 Jun 20;496(6):802-18
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5510438
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Gene symbol:
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Phox2b
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Gene name:
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paired-like homeobox 2b
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Probe:
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Phox2b probe5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2C:
embryonic day 9.5
(more  )
Specimen
2C:
(close  )
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Genetic Background:
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Swiss
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: rhombomere 1 |
Present |
Regionally restricted |
2C
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Expression is as an dispersed longitudinal band.
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| TS15: rhombomere 2 |
Present |
Regionally restricted |
2C
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Expression is in a ventricular longitudinal band in the alar plate.
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| TS15: rhombomere 3 |
Present |
Regionally restricted |
2C
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Expression is in a ventricular longitudinal band in the alar plate.
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| TS15: rhombomere 4 |
Present |
Regionally restricted |
2C
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Expression is in a ventricular longitudinal band in the alar plate.
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| TS15: rhombomere 5 |
Present |
Regionally restricted |
2C
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Expression is in a ventricular longitudinal band in the alar plate.
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| TS15: rhombomere 6 |
Present |
Regionally restricted |
2C
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Expression is in a ventricular longitudinal band in the alar plate.
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Specimen
2D:
embryonic day 10.5
(more )
Specimen
2D:
(close )
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Genetic Background:
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Swiss
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: rhombomere 1 mantle layer |
Strong |
Regionally restricted |
2D
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Expression is in a group of cells at the boundary of rhombomere 1 and 2. Expression is as an dispersed longitudinal band in rhombomere 1.
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| TS17: rhombomere 2 mantle layer |
Strong |
Regionally restricted |
2D
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Expression is in a group of cells at the boundary of rhombomere 1 and 2. Expression is as an dispersed longitudinal band in rhombomere 1.
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Specimen
2H:
embryonic day 11.5
(more )
Specimen
2H:
(close )
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Genetic Background:
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Swiss
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindbrain |
Present |
Regionally restricted |
2H
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Expression is in a ventrally located band of cells in the area of rhombomere 1.
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Specimen
2L:
embryonic day 12.5
(more )
Specimen
2L:
(close )
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Genetic Background:
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Swiss
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindbrain alar plate |
Weak |
Regionally restricted |
2L
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Expression is in a ventrally located band of cells in the area of rhombomere 1.
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| TS20: hindbrain |
Present |
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2L
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Expression is in a group of cells at the boundary of rhombomere 1 and 2.
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Specimen
2O:
embryonic day 11.5
(more )
Note: Vibratome section.
Specimen
2O:
(close )
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Genetic Background:
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Swiss
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Vibratome section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindbrain |
Present |
Regionally restricted |
2O
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Expression is in a ventrally located band of cells in the area of rhombomere 1, the locus coeruleus stream and in a population of cells located between the ventricular longitudinal band in the alar plate and the stream.
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Specimen
2P:
embryonic day 13.5
(more )
Note: Vibratome section.
Specimen
2P:
(close )
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Genetic Background:
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Swiss
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Vibratome section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hindbrain |
Present |
Regionally restricted |
2P
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Expression is in a ventrally located band of cells in the area of rhombomere 1, the locus coeruleus stream and in a population of cells located between the ventricular longitudinal band in the alar plate and the stream.
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Specimen
not shown:
embryonic day 18.5
(more )
Note: Vibratome section.
Specimen
not shown:
(close )
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Genetic Background:
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Swiss
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Vibratome section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hindbrain |
Present |
Regionally restricted |
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Expression is in the locus coeruleus.
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