Pou4f2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:183957 Wu F, et al., Onecut 1 and Onecut 2 are potential regulators of mouse retinal development. J Comp Neurol. 2012 Apr 1;520(5):952-69
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5509319
Gene symbol:	Pou4f2
Gene name:	POU domain, class 4, transcription factor 2

Antibody:	anti-Pou4f2 (C-13) sc-6026
Detection system:	Secondary antibody

Results

3C ©

3C' ©

3C''' ©

Specimen 3C,C',C''': embryonic day 12.5 (more

)
Note: Double labeled: magenta - Pou4f2; green - Onecut1 (assay MGI:5509316).

Structure	Level	Pattern	Image	Note
TS20: retina	Present	Regionally restricted	3C''' , 3C , 3C'	All retinal ganglion cells in the ganglion cell layer expressed both Pou4f2 and Oc1. In the neuroblast layer Pou4f2-positive cells expressed Oc1, but only a subset of Oc1-positive cells also expressed Pou4f2.

3D ©

3D' ©

3D''' ©

Specimen 3D,D',D''': embryonic day 14.5 (more

)
Note: Double labeled: magenta - Pou4f2; green - Onecut1 (assay MGI:5509316).

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted	3D''' , 3D , 3D'	All retinal ganglion cells in the ganglion cell layer expressed both Pou4f2 and Oc1. Overlap in expression in the neuroblast layer decreased.

Specimen not shown: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted		Expression was detected in ganglion cells but not in horizontal cell precursors.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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