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Gene Expression Data
Assay Details
Assay
Reference: J:155251 Lohi M, et al., Expression of Axin2 indicates a role for canonical Wnt signaling in development of the crown and root during pre- and postnatal tooth development. Dev Dyn. 2009 Aug 3;239(1):160-167
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5509162
Gene symbol: Axin2
Gene name: axin 2
Reporter: lacZ
Direct Detection
Results
Specimen 1A: embryonic day 10.5; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS17: 1st branchial arch mandibular component ectoderm Present 1A Expressed in the epithelium.
TS17: 1st branchial arch maxillary component ectoderm Present 1A Expressed in the epithelium.
TS17: 1st branchial arch mandibular component mesenchyme Present 1A
TS17: 1st branchial arch maxillary component mesenchyme Present 1A

Specimen 1B: embryonic day 10.5; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS17: 1st branchial arch mandibular component ectoderm Present 1B Expressed in the epithelium.
TS17: 1st branchial arch mandibular component mesenchyme Present 1B

Specimen 1C: embryonic day 11.5; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS19: latero-nasal process Present Regionally restricted 1C Expressed in both the epithelium and mesenchyme of the lateral nasal processes.
TS19: medial-nasal process Absent 1C

Specimen 1D: embryonic day 11.5; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS19: mandibular process Present Regionally restricted 1D Expressed in both the epithelium and mesenchyme.
TS19: mandibular process mesenchyme Present 1D

Specimen 1E: embryonic day 13.5; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS21: incisor epithelium Present Regionally restricted 1E Expressed as a spherical ball at the anterior part of the tooth bud, where the enamel knot forms.
TS21: incisor mesenchyme Present Regionally restricted 1E Expressed in the condensing mesenchyme.
TS21: vibrissa Present 1E

Specimen 1F: embryonic day 13.5; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS21: incisor epithelium Present Regionally restricted 1F Expressed as a spherical ball at the anterior part of the tooth bud, where the enamel knot forms.
TS21: incisor mesenchyme Present Regionally restricted 1F Expressed in the condensing mesenchyme.

Specimen 1G: embryonic day 13.5; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS21: molar epithelium Present Regionally restricted 1G Expressed in the developing enamel knots.
TS21: molar mesenchyme Present Regionally restricted 1G Expressed in the mesenchyme surrounding the developing enamel knots.

Specimen 1H: embryonic day 13.5; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS21: molar epithelium Present Regionally restricted 1H Expressed in the developing enamel knots.
TS21: molar mesenchyme Present Regionally restricted 1H Expressed in the mesenchyme surrounding the developing enamel knots.

Specimen 1I: embryonic day 14.5; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS22: enamel knot Strong 1I
TS22: tooth mesenchyme Weak Regionally restricted 1I Expressed in the mesenchyme directly beneath the enamel knots.

Specimen 1J: embryonic day 14.5; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS22: enamel knot Strong 1J
TS22: tooth mesenchyme Weak Regionally restricted 1J Expressed in the mesenchyme directly beneath the enamel knots.

Specimen 1K: embryonic day 15.5; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS23: enamel organ Present Diffuse 1K Expressed over a more diffuse area.
TS23: dental papilla Present Regionally restricted 1K Expressed in the mesenchyme underlying the enamel organ.

Specimen 1L: embryonic day 18.5; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS26: secondary enamel knot Present 1L
TS26: stellate reticulum Present 1L
TS26: molar mesenchyme Present Regionally restricted 1L Expressed in the odontoblasts underlying the enamel organ.

Specimen 2A: postnatal day 7; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS28: molar enamel organ Present Regionally restricted 2A Expressed at the tips of the cusps in the region of the enamel-free zone.

Specimen 2B: postnatal day 7; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS28: molar enamel organ Present Regionally restricted 2B Expressed in the cells sitting in the trough on the top of the cusps, with expression absent from the ameloblast cells surrounding the enamel on either side.

Specimen 2C: postnatal day 10; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS28: molar enamel organ Present Regionally restricted 2C Expressed in the cells sitting in the trough on the top of the cusps, with expression absent from the ameloblast cells surrounding the enamel on either side.

Specimen 2D: postnatal day 10; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS28: molar dental pulp Present Regionally restricted 2D Expressed in the developing odontoblasts.

Specimen 2E: postnatal day 12; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS28: molar dental pulp Strong Regionally restricted 2E Expressed in the odontoblasts.

Specimen 2F: postnatal day 15; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS28: molar dental pulp Present Regionally restricted 2F Expression in the odontoblasts started to diminish from cells nearest to the cusps.

Specimen 3A: postnatal day 10; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS28: molar enamel organ Strong Regionally restricted 3A Expressed in the developing roots, concentrated around the root sheath.
TS28: molar dental papilla Strong Regionally restricted 3A Expressed in the root forming regions.

Specimen 3B: postnatal day 10; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS28: molar enamel organ Present Regionally restricted 3B Expressed in the developing roots, concentrated around the root sheath.
TS28: molar dental papilla Present Regionally restricted 3B Expressed in the root forming regions.

Specimen 3C: postnatal day 12; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS28: Hertwig's epithelial root sheath Present 3C
TS28: molar dental papilla Present 3C
TS28: molar dental pulp Present Regionally restricted 3C Expressed in the odontoblasts.

Specimen 3D: postnatal day 12; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS28: Hertwig's epithelial root sheath Present 3D
TS28: molar dental papilla Present 3D
TS28: molar dental pulp Present Regionally restricted 3D Expressed in the odontoblasts.

Specimen 3E: postnatal day 15; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS28: molar root Strong 3E
TS28: molar dental pulp Present Regionally restricted 3E Reduced expression in the odontoblasts.

Specimen 3F: postnatal day 15; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS28: molar enamel organ Strong Regionally restricted 3F Expressed as a V-shape in the inter-radicular region between the roots.

Specimen 5A: postnatal day 3; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS27: incisor dental papilla Weak Regionally restricted 5A Expressed in the dental follicle that surrounds the tooth.
TS27: incisor enamel organ Strong Regionally restricted 5A Expressed in the nonameloblast epithelium adjacent to the dentine. No expression in the ameloblast layer.
TS27: incisor dental pulp Weak Regionally restricted 5A Expressed in the odontoblasts.

Specimen 5B: postnatal day 3; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS27: dental sac Present Regionally restricted 5B Expressed in the mesenchymal tissue surrounding the incisor.
TS27: incisor enamel organ Present Regionally restricted 5B Expressed in the nonameloblast epithelium adjacent to the dentine. No expression in the ameloblast layer.

Specimen 5C: postnatal day 3; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS27: incisor enamel organ Strong Regionally restricted 5C Expressed in the epithelial cells at the very tip. Expressed in the nonameloblast epithelium adjacent to the dentine. No expression in the ameloblast layer.
TS27: dental sac Present Regionally restricted 5C Expressed in the mesenchymal tissue surrounding the incisor.
TS27: incisor dental pulp Weak Regionally restricted 5C Expressed in the odontoblasts.

Specimen 5D: postnatal day 3; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS27: incisor enamel organ Strong Regionally restricted 5D Expressed in the nonameloblast epithelium adjacent to the dentine.
TS27: dental sac Present Regionally restricted 5D Expressed in the mesenchymal tissue surrounding the incisor.

Specimen 5E: postnatal day 15; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS28: cervical loop Strong Regionally restricted 5E Expressed in the odontoblasts at the cervical loop.

Specimen 5F: postnatal day 15; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS28: cervical loop Present Regionally restricted 5F Expressed in the odontoblasts at the cervical loop. No expression in the odonoblasts at the tip.

Specimen 5G: postnatal day 15; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS28: jaw bone Present Regionally restricted 5G Expressed in the bone surrounding the tooth.
TS28: dental sac Present 5G Expressed in the mesenchyme.

Specimen 5H: postnatal day 15; Axin2tm1Wbm/Axin2+ (more )
Structure Level Pattern Image Note
TS28: dental sac Present 5H Expressed in the mesenchyme.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory