Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:83754
Jacquemin P, et al., The Onecut transcription factor HNF-6 (OC-1) is required for timely specification of the pancreas and acts upstream of Pdx-1 in the specification cascade. Dev Biol. 2003 Jun 1;258(1):105-16
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:5508773
|
|
Gene symbol:
|
Pdx1
|
|
Gene name:
|
pancreatic and duodenal homeobox 1
|
|
Antibody:
|
anti-Pdx-1
|
|
Detection system:
|
Secondary antibody coupled to horseradish peroxidase
|
|
|
Results
|
Specimen
1A:
embryonic day 17.5
(more  )
Note: The genotype is not specified. The embryo could be either wild type or heterozygous.
Specimen
1A:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
The genotype is not specified. The embryo could be either wild type or heterozygous.
|
Specimen
1B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Onecut1tm1Flem/Onecut1tm1Flem
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: pancreas |
Present |
|
1B
|
The pancreas was much smaller than in control embryos.
|
Specimen
1C:
embryonic day 15.5
(more )
Note: The genotype is not specified. The embryo could be either wild type or heterozygous.
Specimen
1C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
The genotype is not specified. The embryo could be either wild type or heterozygous.
|
Specimen
1D:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Onecut1tm1Flem/Onecut1tm1Flem
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pancreas |
Present |
|
1D
|
The pancreas was much smaller than in control embryos.
|
Specimen
1E:
embryonic day 12.5
(more )
Note: The genotype is not specified. The embryo could be either wild type or heterozygous.
Specimen
1E:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
The genotype is not specified. The embryo could be either wild type or heterozygous.
|
Specimen
1F:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Onecut1tm1Flem/Onecut1tm1Flem
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pancreas |
Present |
|
1F
|
The pancreatic buds were smaller and less lobulated than in control embryos.
|
Specimen
1G:
embryonic day 10.75
(more )
Note: 37 somites. The genotype is not specified. The embryo could be either wild type or heterozygous.
Specimen
1G:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.75
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
37 somites. The genotype is not specified. The embryo could be either wild type or heterozygous.
|
Specimen
1H:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Onecut1tm1Flem/Onecut1tm1Flem
|
|
Age:
|
embryonic day 10.75
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
37 somites.
|
Specimen
3A:
embryonic day 9.0
(more )
Note: 16 somites. The genotype is not specified. The embryo could be either wild type or heterozygous.
Specimen
3A:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
16 somites. The genotype is not specified. The embryo could be either wild type or heterozygous.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: foregut-midgut junction |
Present |
Regionally restricted |
3A
|
Expressed in the dorsal and ventral parts of the gut endoderm.
|
Specimen
3B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Onecut1tm1Flem/Onecut1tm1Flem
|
|
Age:
|
embryonic day 9.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
16 somites.
|
Specimen
3C:
embryonic day 9.5
(more )
Note: 19 somites. The genotype is not specified. The embryo could be either wild type or heterozygous.
Specimen
3C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
19 somites. The genotype is not specified. The embryo could be either wild type or heterozygous.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: foregut-midgut junction |
Present |
Regionally restricted |
3C
|
Expressed in the dorsal and ventral parts of the gut endoderm.
|
Specimen
3D:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Onecut1tm1Flem/Onecut1tm1Flem
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
19 somites.
|
Specimen
3E:
embryonic day 9.5
(more )
Note: 22 somites. The genotype is not specified. The embryo could be either wild type or heterozygous.
Specimen
3E:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
22 somites. The genotype is not specified. The embryo could be either wild type or heterozygous.
|
Specimen
3F:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Onecut1tm1Flem/Onecut1tm1Flem
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
22 somites.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: foregut-midgut junction |
Present |
Regionally restricted |
3F
|
Expressed in the dorsal region of the gut endoderm, with a much lower positive cell number than in control. Only two positive cells in the ventral part of the endoderm.
|
Specimen
4B:
embryonic day 8.5
(more )
Note: 8 somites. The genotype is not specified. The embryo could be either wild type or heterozygous.
Specimen
4B:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
8 somites. The genotype is not specified. The embryo could be either wild type or heterozygous.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: gut endoderm |
Present |
Regionally restricted |
4B
|
Expressed in a few cells of the endodermal lip, which will give rise to the ventral pancreatic bud. No expression in the dorsal endoderm.
|
Specimen
4D:
embryonic day 9.0
(more )
Note: 13 somites. The genotype is not specified. The embryo could be either wild type or heterozygous.
Specimen
4D:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
13 somites. The genotype is not specified. The embryo could be either wild type or heterozygous.
|
Specimen
4F:
embryonic day 9.0
(more )
Note: 13 somites. The genotype is not specified. The embryo could be either wild type or heterozygous.
Specimen
4F:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
13 somites. The genotype is not specified. The embryo could be either wild type or heterozygous.
|
|
Analysis Tools