Probe:	Pod1/Capsulin probe1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS21: ovary	Present		1B F	Expression was stronger in the male gonad than in the female.

Structure	Level	Pattern	Image	Note
TS21: testis	Strong		1B M	Expression was stronger in the male gonad than in the female.

Structure	Level	Pattern	Image	Note
TS15: aorta-gonad-mesonephros	Present	Regionally restricted	3A	No difference in expression was noted between the male and female.

Structure	Level	Pattern	Image	Note
TS17: urogenital ridge	Strong		3C
TS17: branchial arch	Present		3C	The level of expression was lower than in the urogenital ridge.
TS17: genitourinary system	Present	Regionally restricted	3C	Expression was detected in the mesentery near the urogenital ridge.
TS17: heart	Present	Regionally restricted	3C	Expression was detected in the epicardium. The level of expression was lower than in the urogenital ridge.

Structure	Level	Pattern	Image	Note
TS19: gonad	Present		3E	No difference in expression was noted between the male and female.

Structure	Level	Pattern	Image	Note
TS21: adrenal gland	Present
TS21: gonad	Present
TS21: heart	Present
TS21: intestine	Present
TS21: lung	Present
TS21: metanephros	Present

Structure	Level	Pattern	Image	Note
TS21: testis	Present	Regionally restricted	4A	Expression was detected in the tunica albuginea.
TS21: interstitium of the testis	Present		4A

Structure	Level	Pattern	Image	Note
TS21: ovary	Weak	Diffuse	4B

Structure	Level	Pattern	Image	Note
TS23: mesonephros	Present		4C	There was no sex-dependent difference in expression in the mesonephros.

Structure	Level	Pattern	Image	Note
TS23: ovary	Present	Regionally restricted	4D	Expression was detected in the female gonad, probably in follicle and/or interstitial cells between oocytes.
TS23: mesonephros	Present		4D	There was no sex-dependent difference in expression in the mesonephros.

Structure	Level	Pattern	Image	Note
TS21: testis	Present	Regionally restricted	4J	Expression was detected in the tunica albuginea.
TS21: interstitium of the testis	Present		4J

Structure	Level	Pattern	Image	Note
TS17: urogenital ridge	Present	Regionally restricted	5A	Expression was detected in the coelomic epithelial cells. Expression did not overlap with Ad4BP/SF-1.
TS17: mesonephros mesenchyme	Present		5A	Expression did not overlap with Ad4BP/SF-1.

Structure	Level	Pattern	Image	Note
TS19: gonad	Present	Regionally restricted	5B	Expression was similar to E10.5. Expression did not overlap with Ad4BP/SF-1.

Structure	Cell Type	Level	Pattern	Image	Note
TS25: testis		Present	Regionally restricted	5C	Expression was detected in the coelomic epithelial cells, peritubular myoid cells and epithelial-like cells. Expression did not overlap with Ad4BP/SF-1.
TS25: peritubular myoid cell	peritubular myoid cell	Present		5C

Structure	Level	Pattern	Image	Note
TS23: ovary	Present			Expression did not overlap with Ad4BP/SF-1.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Sertoli cell	Sertoli cell	Present		6A

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Sertoli cell	Sertoli cell	Absent			No expression was detected in Sertoli cells at postnatal day 30.

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present
TS28: ovary follicle	Present	Regionally restricted		Expression was detected in granulosa cells.