Gene Expression Data
Assay Details
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Assay
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Reference:
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J:34199
Pringle NP, et al., Determination of neuroepithelial cell fate: induction of the oligodendrocyte lineage by ventral midline cells and sonic hedgehog. Dev Biol. 1996 Jul 10;177(1):30-42
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5506520
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Gene symbol:
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Pdgfra
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Gene name:
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platelet derived growth factor receptor, alpha polypeptide
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Probe:
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Pdgfra probe8
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
1a:
embryonic day 13.0; Etn2Sd/Etn2 +
(more  )
Note: Caudal-most section in the spinal cord, 2mm from the tip of the tail.
Specimen
1a:
(close  )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Etn2Sd/Etn2+
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Caudal-most section in the spinal cord, 2mm from the tip of the tail.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: embryo |
Present |
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1a
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Expression was detected in many tissues outside of the central nervous system.
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| TS21: spinal cord ventricular layer |
Present |
Regionally restricted |
1a
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Expression was detected in bilateral foci in the ventral ventricular zone in regions of the spinal cord that were associated with a floor plate.
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Specimen
1b:
embryonic day 13.0; Etn2Sd/Etn2 +
(more )
Note: More anterior than 1a.
Specimen
1b:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Etn2Sd/Etn2+
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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More anterior than 1a.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: embryo |
Present |
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1b
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Expression was detected in many tissues outside of the central nervous system.
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| TS21: spinal cord ventricular layer |
Absent |
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1b
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Where there was no floor plate, there was no expression in that region of the spinal cord.
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Specimen
1c:
embryonic day 13.0; Etn2Sd/Etn2 +
(more )
Note: More anterior than 1b.
Specimen
1c:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Etn2Sd/Etn2+
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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More anterior than 1b.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: embryo |
Present |
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1c
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Expression was detected in many tissues outside of the central nervous system.
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| TS21: spinal cord ventricular layer |
Present |
Regionally restricted |
1c
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Expression was detected in bilateral foci in the ventral ventricular zone in regions of the spinal cord that were associated with a floor plate.
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Specimen
2A:
embryonic day 13.0; Etn2Sd/Etn2 +
(more )
Note: Section from the caudal region with a floor plate.
Specimen
2A:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Etn2Sd/Etn2+
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Section from the caudal region with a floor plate.
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Specimen
2B:
embryonic day 13.0; Etn2Sd/Etn2 +
(more )
Note: Section from the caudal region without a floor plate.
Specimen
2B:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Etn2Sd/Etn2+
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Section from the caudal region without a floor plate.
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Specimen
3a:
embryonic day 15.0; Etn2Sd/Etn2 +
(more )
Note: Section from the caudal region, lacking a floor plate.
Specimen
3a:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Etn2Sd/Etn2+
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Section from the caudal region, lacking a floor plate.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: spinal cord |
Absent |
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3a
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There were regions of the spinal cord that lacked a floor plate that also lacked expression.
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Specimen
3b:
embryonic day 15.0; Etn2Sd/Etn2 +
(more )
Note: Section from the caudal region, lacking a floor plate.
Specimen
3b:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Etn2Sd/Etn2+
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Section from the caudal region, lacking a floor plate.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: spinal cord |
Present |
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3b
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There were regions of the spinal cord that lacked a floor plate, but expression was detected in dispersed cells.
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| TS23: spinal cord ventricular layer |
Absent |
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3b
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If there were expressing cells dispersed in the spinal cord section, they were not found at the ventricular surface.
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Specimen
3c:
embryonic day 15.0; Etn2Sd/Etn2 +
(more )
Note: More anterior section than 3b, possesses a floor plate.
Specimen
3c:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Etn2Sd/Etn2+
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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More anterior section than 3b, possesses a floor plate.
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Analysis Tools