Gene Expression Data
Assay Details
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Assay
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Reference:
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J:84057
Lee HO, et al., The endothelin receptor-B is required for the migration of neural crest-derived melanocyte and enteric neuron precursors. Dev Biol. 2003 Jul 1;259(1):162-75
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5505800
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Gene symbol:
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Dct
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Gene name:
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dopachrome tautomerase
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Probe:
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Dct cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2G:
embryonic day 12.5; Ednrbtm1Myks/Ednrb +
(more  )
Specimen
2G:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ednrbtm1Myks/Ednrb+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: skin |
Present |
Regionally restricted |
2G
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Expressed in migrating melanoblasts.
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Specimen
2H:
embryonic day 12.5; Ednrbtm1Myks/Ednrb +
(more )
Specimen
2H:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ednrbtm1Myks/Ednrb+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: skin |
Present |
Regionally restricted |
2H
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Expressed in migrating melanoblasts.
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Specimen
3F/I:
embryonic day 11.5; Ednrbtm1Myks/Ednrb +
(more )
Specimen
3F/I:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ednrbtm1Myks/Ednrb+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: mesenchyme derived from neural crest |
Present |
Regionally restricted |
3I
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3F
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Expressed in migrating melanoblasts throughout the rostral-caudal axis. Migrating melanoblasts have reached the base of the hindlimb.
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Specimen
3G/J:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ednrbtm1Myks/Ednrbtm1Myks
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: mesenchyme derived from neural crest |
Present |
Regionally restricted |
3G
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3J
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Expressed in migrating melanoblasts in the caudal portion. Almost no melanoblasts were observed in the rostral portion. Migration stalled at the dorsal end of the dermomyotome.
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Specimen
3H/K:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ednrbtm1Myks/Ednrbtm1Myks
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: mesenchyme derived from neural crest |
Present |
Regionally restricted |
3H
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3K
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Expressed in migrating melanoblasts in the caudal portion. Almost no melanoblasts were observed in the rostral portion. Migration stalled at the dorsal end of the dermomyotome.
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Specimen
4A:
embryonic day 10.0; Ednrbtm1Myks/Ednrb +
(more )
Note: 29 somites embryo.
Specimen
4A:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ednrbtm1Myks/Ednrb+
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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29 somites embryo.
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Specimen
4B:
embryonic day 10.0; Ednrbtm1Myks/Ednrb +
(more )
Note: 29 somites embryo.
Specimen
4B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ednrbtm1Myks/Ednrb+
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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29 somites embryo.
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Specimen
4C:
embryonic day 10.0; Ednrbtm1Myks/Ednrb +
(more )
Note: 29 somites embryo.
Specimen
4C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ednrbtm1Myks/Ednrb+
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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29 somites embryo.
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Specimen
4D:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ednrbtm1Myks/Ednrbtm1Myks
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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27 somites embryo.
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Specimen
4E:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ednrbtm1Myks/Ednrbtm1Myks
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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27 somites embryo.
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Specimen
4F:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ednrbtm1Myks/Ednrbtm1Myks
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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27 somites embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: embryo |
Present |
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4F
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Expressed in melanoblast cells throughout the rostral-caudal axis. Decreased expression compared with that of the heterozygous.
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Specimen
4G:
embryonic day 10.5; Ednrbtm1Myks/Ednrb +
(more )
Note: 35 somites embryo.
Specimen
4G:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ednrbtm1Myks/Ednrb+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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35 somites embryo.
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Specimen
4H:
embryonic day 10.5; Ednrbtm1Myks/Ednrb +
(more )
Note: 35 somites embryo.
Specimen
4H:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ednrbtm1Myks/Ednrb+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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35 somites embryo.
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Specimen
4I:
embryonic day 10.5; Ednrbtm1Myks/Ednrb +
(more )
Note: 35 somites embryo.
Specimen
4I:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ednrbtm1Myks/Ednrb+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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35 somites embryo.
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Specimen
4J:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ednrbtm1Myks/Ednrbtm1Myks
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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35 somites embryo.
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Specimen
4K:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ednrbtm1Myks/Ednrbtm1Myks
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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35 somites embryo.
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Specimen
4L:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ednrbtm1Myks/Ednrbtm1Myks
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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35 somites embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: embryo |
Present |
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4L
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Expressed in melanoblast cells throughout the rostral-caudal axis. Decreased expression compared with that of the heterozygous.
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Specimen
4M:
embryonic day 11.0
(more )
Note: 40 somites embryo.
Specimen
4M:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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40 somites embryo.
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Specimen
4N:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ednrbtm1Myks/Ednrbtm1Myks
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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42 somites embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: trunk mesenchyme derived from neural crest |
Present |
Regionally restricted |
4N inset
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4N
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Expressed in melanoblast cells along the rostral-caudal axis, but no melanoblasts present between the otic vesicle and forelimb. Expressed in melanoblasts between the forelimb and mid-trunkal area. Decreased expression compared with that of the wild type.
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