Gene Expression Data
Assay Details
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Assay
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Reference:
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J:83697
Weaver M, et al., Tissue interactions pattern the mesenchyme of the embryonic mouse lung. Dev Biol. 2003 Jun 1;258(1):169-84
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5505680
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Gene symbol:
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Nog
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Gene name:
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noggin
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
1E:
embryonic day 11.5; Nogtm1Amc/Nog +
(more  )
Specimen
1E:
(close  )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Nogtm1Amc/Nog+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: lung mesenchyme |
Strong |
Regionally restricted |
1E
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Strong expression was restricted to one or two cell layers immediately adjacent to the proximal epithelium.
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Specimen
1F,G:
embryonic day 11.5; Nogtm1Amc/Nog +
(more )
Specimen
1F,G:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Nogtm1Amc/Nog+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: lung mesenchyme |
Strong |
Regionally restricted |
1F
,
1G
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Strong expression was restricted to one or two cell layers immediately adjacent to the proximal epithelium.
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Specimen
H:
embryonic day 11.0; Nogtm1Amc/Nog +
(more )
Specimen
H:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Nogtm1Amc/Nog+
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: lung mesenchyme |
Weak |
Regionally restricted |
1H
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Low, 'salt-and-pepper' staining was detected in distal mesenchyme.
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Specimen
1L,M:
embryonic day 13.5; Nogtm1Amc/Nog +
(more )
Specimen
1L,M:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Nogtm1Amc/Nog+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1N:
embryonic day 13.5; Nogtm1Amc/Nog +
(more )
Specimen
1N:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Nogtm1Amc/Nog+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: lung mesenchyme |
Present |
Regionally restricted |
1N
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Expression was restricted to cells directly adjacent to proximal epithelium.
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Specimen
2C,E:
embryonic day 13.5; Nogtm1Amc/Nog +
(more )
Specimen
2C,E:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Nogtm1Amc/Nog+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: smooth muscle tissue |
Present |
Regionally restricted |
2E
,
2C
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Expression was detected in smooth muscle adjacent to the proximal endoderm.
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| TS21: lung mesenchyme |
Present |
Regionally restricted |
2E
,
2C
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Expression was detected in a 'salt-and-pepper' distribution.
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Specimen
2G:
embryonic day 13.5; Nogtm1Amc/Nog +
(more )
Specimen
2G:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Nogtm1Amc/Nog+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2I,J:
embryonic day 13.5; Nogtm1Amc/Nog +
(more )
Note: Double labeled: blue - Nog reporter; purple - Pecam1 (assay MGI:5505703).
Specimen
2I,J:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Nogtm1Amc/Nog+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: blue - Nog reporter; purple - Pecam1 (assay MGI:5505703).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: blood vessel |
Absent |
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2J
,
2I
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Staining was not detected in blood vessels or capillary beds.
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| TS21: smooth muscle tissue |
Present |
Regionally restricted |
2J
,
2I
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Expression was detected in parabronchial smooth muscle, but not in perivascular smooth muscle.
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Specimen
not shown E11.5:
embryonic day 11.5; Nogtm1Amc/Nog +
(more )
Specimen
not shown E11.5:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Nogtm1Amc/Nog+
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Age:
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embryonic day 11.5
|
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: lung mesenchyme |
Present |
Regionally restricted |
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Expression was detected in parabronchial smooth muscle.
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Specimen
not shown E14.5:
embryonic day 14.5; Nogtm1Amc/Nog +
(more )
Specimen
not shown E14.5:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Nogtm1Amc/Nog+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: smooth muscle tissue |
Present |
Regionally restricted |
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Expression was detected in parabronchial smooth muscle.
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Specimen
not shown E15.5:
embryonic day 15.5; Nogtm1Amc/Nog +
(more )
Specimen
not shown E15.5:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Nogtm1Amc/Nog+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: smooth muscle tissue |
Present |
Regionally restricted |
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Expression was detected in parabronchial smooth muscle.
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