Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:83697
Weaver M, et al., Tissue interactions pattern the mesenchyme of the embryonic mouse lung. Dev Biol. 2003 Jun 1;258(1):169-84
|
|
Assay type:
|
In situ reporter (knock in)
|
|
MGI Accession ID:
|
MGI:5505676
|
|
Gene symbol:
|
Bmp4
|
|
Gene name:
|
bone morphogenetic protein 4
|
|
Reporter:
|
lacZ
|
|
|
Direct Detection
|
|
|
Results
|
Specimen
1A:
embryonic day 11.5; Bmp4tm2Blh/Bmp4 +
(more  )
Specimen
1A:
(close  )
|
Genetic Background:
|
involves: 129S6/SvEvTac * Black Swiss
|
|
Mutant Allele(s):
|
Bmp4tm2Blh/Bmp4+
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: lung mesenchyme |
Strong |
Regionally restricted |
1A
|
Expression was seen in a wide swath of cells, with highest levels adjacent to the proximal epithelium and lower levels toward the periphery.
|
Specimen
1B,C,D:
embryonic day 11.5; Bmp4tm2Blh/Bmp4 +
(more )
Specimen
1B,C,D:
(close )
|
Genetic Background:
|
involves: 129S6/SvEvTac * Black Swiss
|
|
Mutant Allele(s):
|
Bmp4tm2Blh/Bmp4+
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: lung mesenchyme |
Strong |
Regionally restricted |
1B
,
1D
,
1C
|
Expression was detected in mesenchyme cells adjacent to main bronchi, proximal endoderm, and in interbud regions.
|
Specimen
1I,J:
embryonic day 13.5; Bmp4tm2Blh/Bmp4 +
(more )
Specimen
1I,J:
(close )
|
Genetic Background:
|
involves: 129S6/SvEvTac * Black Swiss
|
|
Mutant Allele(s):
|
Bmp4tm2Blh/Bmp4+
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: lung mesenchyme |
Present |
|
1I
,
1J
|
The overall expression patterns are maintained at E13.5.
|
Specimen
1K:
embryonic day 13.5; Bmp4tm2Blh/Bmp4 +
(more )
Specimen
1K:
(close )
|
Genetic Background:
|
involves: 129S6/SvEvTac * Black Swiss
|
|
Mutant Allele(s):
|
Bmp4tm2Blh/Bmp4+
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: lung mesenchyme |
Present |
Graded |
1K
|
Highest expression was adjacent to epithelium decreasing toward the periphery.
|
Specimen
2A:
embryonic day 13.5; Bmp4tm2Blh/Bmp4 +
(more )
Specimen
2A:
(close )
|
Genetic Background:
|
involves: 129S6/SvEvTac * Black Swiss
|
|
Mutant Allele(s):
|
Bmp4tm2Blh/Bmp4+
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: lung mesenchyme |
Present |
Regionally restricted |
2A
|
Expression was detected in a wide domain, including cells adjacent to the endoderm.
|
| TS21: smooth muscle tissue |
Present |
Regionally restricted |
2A
|
Expression was detected in parabronchial smooth muscle.
|
|
Analysis Tools
