Myl1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:77516 Jiang P, et al., Targeted deletion of the MLC1f/3f downstream enhancer results in precocious MLC expression and mesoderm ablation. Dev Biol. 2002 Mar 15;243(2):281-93
Assay type:	RNA in situ
MGI Accession ID:	MGI:5501216
Gene symbol:	Myl1
Gene name:	myosin, light polypeptide 1

Probe:	MLC3f cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	The fixative was MEMFA (0.1 M Mops, pH 7.5, 2 mM EGTA, 1 mM MgSO4, 3.7% formaldehyde).

Results

3e +/+ ©

Specimen 3e +/+: embryonic day 7.5 (more

)

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent		3e +/+

3e +/- ©

Specimen 3e +/-: embryonic day 7.5; Myl1^tm1Nros/Myl1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent		3e +/-

3e -/- ©

Specimen 3e -/-: embryonic day 7.5; Myl1^tm1Nros/Myl1^tm1Nros (more

)

Structure	Level	Pattern	Image	Note
TS11: embryo	Present		3e -/-

3f normal ©

Specimen 3f normal: embryonic day 7.5 (more

)

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent		3f normal

3f mutant ©

Specimen 3f mutant: embryonic day 7.5; Myl1^tm1Nros/Myl1^tm1Nros (more

)

Structure	Level	Pattern	Image	Note
TS11: embryo	Present		3f mutant

Specimen 4 MLCE-/-: embryonic day 7.5; Myl1^tm1Nros/Myl1^tm1Nros (more

)

Structure	Level	Pattern	Image	Note
TS11: embryo	Present		4_MLCE-/- mutant

Specimen 4 E7.5 wild type: embryonic day 7.5 (more

)

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent		4_Wild-type E7.5

Specimen 4 E8.5: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: heart	Present		4_Wild-type E8.5

Specimen 4 E9.5: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: heart	Present		4_Wild-type E9.5
TS15: somite	Present		4_Wild-type E9.5

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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