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Gene Expression Data
Assay Details
Assay
Reference: J:180855 Briot A, et al., Par2 inactivation inhibits early production of TSLP, but not cutaneous inflammation, in Netherton syndrome adult mouse model. J Invest Dermatol. 2010 Dec;130(12):2736-42
Assay type: RT-PCR
MGI Accession ID: MGI:5496528
Gene symbol: Tnf
Gene name: tumor necrosis factor
Probe: Tnf alpha-pA, Tnf alpha-pB
Probe preparation: labelled with SYBR green
Assay notes: Quantitative real-time PCR. Results were normalized with the Hprt gene.
Results Image: 1e Tnf alpha
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
WT E19.5 TS26: skin Ambiguous 2-3 µg; total RNA Not Specified Not Specified
Spink5-/- E19.5 TS26: skin Present (a) 2-3 µg; total RNA involves: 129P2/OlaHsd * FVB Spink5tm1Hov/Spink5tm1HovNot Specified
Par2-/- E19.5 TS26: skin Ambiguous 2-3 µg; total RNA involves: 129S4/SvJae F2rl1tm1Cgh/F2rl1tm1CghNot Specified
DKO E19.5 TS26: skin Present (b) 2-3 µg; total RNA involves: 129P2/OlaHsd * 129S4/SvJae F2rl1tm1Cgh/F2rl1tm1Cgh, Spink5tm1Hov/Spink5tm1HovNot Specified
Notes:
(a) There was a 26.6-fold induction relative to wild type in the Spink5-/- skin.
(b) There was a 15.8-fold induction relative to wild type in the DKO skin. The increase in the DKO was 60% of the increase seen in the Spink5-/- skin.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory