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Gene Expression Data
Assay Details
Assay
Reference: J:110707 Pattyn A, et al., Delays in neuronal differentiation in Mash1/Ascl1 mutants. Dev Biol. 2006 Jul 1;295(1):67-75
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5495264
Gene symbol: Phox2b
Gene name: paired-like homeobox 2b
Antibody: anti-Phox2b
Detection system: horseradish peroxidase
Results
Specimen 1A: embryonic day 10.5 (more )
Note: Double labeled: orange - Phox2b; dark blue - Ascl1 (assay MGI:5495268).
Structure Level Pattern Image Note
TS17: hindbrain Present Regionally restricted 1A Expression in nucleus of solitary tract (nTS) precursors.
Specimen 1B: embryonic day 11.5 (more )
Note: Double labeled: orange - Phox2b; dark blue - Ascl1 (assay MGI:5495268).
Structure Level Pattern Image Note
TS19: hindbrain Present Regionally restricted 1B Expression in ventrally-generated motoneurons migrating dorsally.
Specimen 2A WT: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: hindbrain Present Regionally restricted 2A WT Expression in nucleus of solitary tract (nTS) and area postrema (ap).
Specimen 2A Mash1-/-: embryonic day 18.5; Ascl1tm1And/Ascl1tm1And (more )
Structure Level Pattern Image Note
TS26: hindbrain Present 2A Mash1-/- Authors state that the nucleus of the solitary tract (nTS) and area postrema (ap) are atrophic.
Specimen 2B WT: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: hindbrain Present Regionally restricted 2B WT Expression in nucleus of solitary tract (nTS) and area postrema (ap).
Specimen 2B Mash1-/-: embryonic day 10.5; Ascl1tm1And/Ascl1tm1And (more )
Structure Level Pattern Image Note
TS17: hindbrain Present Regionally restricted 2B Mash1-/- Authors state that nucleus of solitary tract precursors (nTSp) are not detected, but ventrally born visceral motoneurons (vMN) are present, although slightly delayed in their dorsal migration.
Specimen 2E WT: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: hindbrain Present Regionally restricted 2E WT Expression in nucleus of solitary tract (nTS) and area postrema (ap).
Specimen 2E Mash1-/-: embryonic day 11.5; Ascl1tm1And/Ascl1tm1And (more )
Structure Level Pattern Image Note
TS19: hindbrain Present Regionally restricted 2E Mash1-/- Few nucleus of solitary tract precursors (nTSp) are detectable, but no dl5 neurons.
Specimen 4A: embryonic day 10.5 (more )
Note: Double labeled: orange - Phox2b; dark blue - Neurog2 (assay MGI:5495270).
Structure Level Pattern Image Note
TS17: hindbrain mantle layer Present Regionally restricted 4A Expression in postmitotic cells in mantle layer.
Specimen 4B WT: embryonic day 12.0 (more )
Structure Level Pattern Image Note
TS20: hindbrain Present 4B WT
Specimen 4B Ngn2-/-: embryonic day 12.0; Neurog2tm1Fgu/Neurog2tm1Fgu (more )
Structure Level Pattern Image Note
TS20: hindbrain Present 4B Ngn2-/- Authors report no obvious phenotype in Ngn2 mutants.
Specimen 4B Mash1-/-;Ngn2-/-: embryonic day 12.0; Ascl1tm1And/Ascl1tm1And, Neurog2tm1Fgu/Neurog2tm1Fgu (more )
Structure Level Pattern Image Note
TS20: hindbrain Present 4B Mash1-/-;Ngn2-/- Authors state that the phenotype of Mash1;Ngn2 double mutants is similar to that of Mash1 single mutants.
Specimen 6A WT: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: sympathetic nervous system Present Regionally restricted 6A WT Phox2b Expression in sympathoblast aggregating around the dorsal aorta.
Specimen 6A Mash1-/-: embryonic day 10.5; Ascl1tm1And/Ascl1tm1And (more )
Structure Level Pattern Image Note
TS17: sympathetic nervous system Present Regionally restricted 6A Mash1-/- Phox2b Expression in sympathoblast aggregating around the dorsal aorta.
Specimen 6B WT: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: thoracic ganglion Present 6B WT Phox2b
Specimen 6B Mash1-/-: embryonic day 11.5; Ascl1tm1And/Ascl1tm1And (more )
Structure Level Pattern Image Note
TS19: thoracic ganglion Present 6B Mash1-/- Phox2b
Specimen 6C WT: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: thoracic ganglion Present 6C WT Phox2b
Specimen 6C Mash1-/-: embryonic day 12.5; Ascl1tm1And/Ascl1tm1And (more )
Structure Level Pattern Image Note
TS20: thoracic ganglion Present 6C Mash1-/- Phox2b
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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