Gene Expression Data
Assay Details
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Assay
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Reference:
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J:171806
Young B, et al., Indian and sonic hedgehogs regulate synchondrosis growth plate and cranial base development and function. Dev Biol. 2006 Nov 1;299(1):272-82
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5493323
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Gene symbol:
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Spp1
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Gene name:
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secreted phosphoprotein 1
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Probe:
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Osteopontin cDNA4
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5C:
embryonic day 15.5
(more  )
Specimen
5C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cranial synchondrosis |
Present |
Regionally restricted |
5C
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Expression was detected in mature hypertrophic chondrocytes at the growth plate end facing endochondral bone. The basi-occipital ossification center also had expression.
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Specimen
5G:
embryonic day 17.5
(more )
Specimen
5G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: cranial synchondrosis |
Present |
Regionally restricted |
5G
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Expression was detected in mature hypertrophic chondrocytes at the growth plate end facing endochondral bone.
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Specimen
5K:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Ihhtm1Amc/Ihhtm1Amc
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cranial synchondrosis |
Present |
Regionally restricted |
5K
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The basi-occipital ossification center had normal expression.
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Specimen
5O:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Ihhtm1Amc/Ihhtm1Amc
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: cranial synchondrosis |
Present |
Regionally restricted |
5O
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The growth plate is mal-organized and the posterior sphenoidal ossification center is occupied by positively stained hypertrophic chondrocytes.
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Specimen
6C:
embryonic day 17.5
(more )
Specimen
6C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: cranial synchondrosis |
Present |
Regionally restricted |
6C
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In intrasphenoidal synchondrosis, expression was detected in hypertrophic chondrocytes abutting the perichondrial tissue.
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Specimen
6I:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Ihhtm1Amc/Ihhtm1Amc
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: cranial synchondrosis |
Present |
Regionally restricted |
6I
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Expression was detected in hypertrophic chondrocytes that tended to occupy a more central location throughout the synchondroses.
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Analysis Tools