Probe:	pSP73-11
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	4A	Expressed in the head mesoderm and the mesodermal layers of the tail. The paraxial mesoderm in the trunk region was devoid of expression.
TS11: embryo ectoderm	Absent		4A
TS11: future spinal cord neural plate	Ambiguous		4A	Little expressed.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	4B	Expressed in the head mesoderm and the mesodermal layers of the tail. The paraxial mesoderm in the trunk region was devoid of expression.
TS11: embryo ectoderm	Absent		4B

Structure	Level	Pattern	Image	Note
TS12: head mesenchyme	Strong	Non-Uniform	4C	Expressed in most of the mesenchymal cells, although the staining intensity varied with the subregion.
TS12: somite	Present		4C
TS12: future spinal cord neural plate	Ambiguous		4C	Little expressed.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch mandibular component mesenchyme	Strong		5A
TS14: 1st branchial arch maxillary component mesenchyme	Strong		5A
TS14: future spinal cord neural fold	Weak	Regionally restricted	5A	Expressed in the ridges.
TS14: somite	Present	Regionally restricted	5A	Expressed at the caudal region of the newly condensing mesenchymal cells. Expressed in the entire somite when the closed epithelial vesicles were formed. At later stages, the dermomyotome gradually lost expression.
TS14: notochord	Absent		5A

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mandibular component mesenchyme	Strong		5C
TS15: 1st branchial arch maxillary component mesenchyme	Strong		5C
TS15: somite	Present	Regionally restricted	5C	Expressed at the caudal region of the newly condensing mesenchymal cells. Expressed in the entire somite when the closed epithelial vesicles were formed. At later stages, the dermomyotome gradually lost expression.
TS15: forelimb bud ectoderm	Absent		5C
TS15: notochord	Absent		5C
TS15: forelimb bud mesenchyme	Present	Regionally restricted	5C	Expressed in the distal region.

Structure	Level	Pattern	Image	Note
TS18: 1st branchial arch mandibular component mesenchyme	Strong		5E
TS18: 1st branchial arch maxillary component mesenchyme	Strong		5E
TS18: somite	Present	Regionally restricted	5E	Expressed at the caudal region of the newly condensing mesenchymal cells. Expressed in the entire somite when the closed epithelial vesicles were formed. At later stages, the dermomyotome gradually lost expression.
TS18: notochord	Absent		5E

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	6A	Expressed in the head mesoderm and the mesodermal layers of the tail.
TS11: embryo ectoderm	Absent		6A

Structure	Level	Pattern	Image	Note
TS14: head mesenchyme	Strong	Non-Uniform	6B	Expressed in most of the mesenchymal cells, although the staining intensity varied with the subregion.
TS14: neural tube roof plate	Present		6B	Expressed in the dorsal midline in the posterior trunk region.

Structure	Level	Pattern	Image	Note
TS14: optic eminence mesenchyme	Present	Regionally restricted	6C	Expressed in the periocular mesenchyme in the outer region of the optic vesicle.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present		6D

Structure	Level	Pattern	Image	Note
TS15: neural tube roof plate	Present		6E	Expressed in the dorsal midline in the posterior trunk region.
TS15: trigeminal preganglion	Absent		6E	No expression in the cranial and dorsal ganglion, presumably the trigeminal ganglion.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud mesenchyme	Present	Graded	6F	Expressed in a distal-proximal gradient with an irregular shape along the anterior-posterior axis.
TS16: forelimb bud apical ectodermal ridge	Absent		6F
TS16: forelimb bud ectoderm	Absent		6F

Structure	Level	Pattern	Image	Note
TS13: somite	Present	Regionally restricted	7A	Expressed at the caudal region of the newly condensing mesenchymal cells. Expressed in the entire somite when the closed epithelial vesicles were formed. At later stages, the dermomyotome gradually lost expression.

Structure	Level	Pattern	Image	Note
TS14: somite	Present	Regionally restricted	7B	Expressed at the caudal region of the newly condensing mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS14: dermomyotome	Absent		7C
TS14: sclerotome	Present		7C

Structure	Level	Pattern	Image	Note
TS14: sclerotome	Present	Non-Uniform	7D	Expressed in the cells that had started migration. Cells at both cranial and caudal edges expressed more than those in the middle region.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Present	Regionally restricted	9A	Expressed in the distal region.
TS15: forelimb bud ectoderm	Absent		9A

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Graded	9B	Expressed in a distal-proximal gradient with an irregular shape along the anterior-posterior axis.

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Strong	Regionally restricted	9C	Expressed in the peripheral areas, extending into deeper layers at each interdigital region.

Structure	Level	Pattern	Image	Note
TS21: hindlimb mesenchyme	Strong	Regionally restricted	9D	Expressed in the peripheral areas.