Probe:	Ank probe1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS23: clavicle	Present	Regionally restricted	1A/A'	Expression in maxilla, mandible and clavicle localized to mesenchymal cells within condensation of bone anlagen.
TS23: mandible	Present	Regionally restricted	1A/A'	Expression in maxilla, mandible and clavicle localized to mesenchymal cells within condensation of bone anlagen.
TS23: maxilla	Present	Regionally restricted	1A/A'	Expression in maxilla, mandible and clavicle localized to mesenchymal cells within condensation of bone anlagen.
TS23: basisphenoid bone	Present	Regionally restricted	1A/A'	Expression in ossification center of basisphenoid bone.
TS23: humerus	Present	Regionally restricted	1A/A'	Expression in ossification center of long bones including the humerus and tibia.
TS23: tibia	Present	Regionally restricted	1A/A'	Expression in ossification center of long bones including the humerus and tibia.
TS23: metatarsus mesenchyme	Absent		1A/A'	No expression in hypertrophic cells in metatarsal before formation of ossification center.
TS23: rib	Present	Regionally restricted	1A/A'	Expression in cells lined up along inner edge of perichondrium/perioseum in areas of bone collar formation near sections of hypertrophic cartilage.
TS23: olfactory epithelium	Present	Regionally restricted	1A/A'	Expression in olfactory epithelium and associated serous glands.
TS23: olfactory lobe	Present		1A/A'

Structure	Level	Pattern	Image	Note
TS23: frontal bone primordium	Present	Regionally restricted	1B/B'	Expression in frontal bone and maxilla, localized to mesenchymal cells within condensation of bone anlagen.
TS23: maxilla	Present	Regionally restricted	1B/B'	Expression in frontal bone and maxilla, localized to mesenchymal cells within condensation of bone anlagen.
TS23: basioccipital bone	Present	Regionally restricted	1B/B'	Expression in ossification centers of basioccipital and basisphenoid bones (endochondral).
TS23: basisphenoid bone	Present	Regionally restricted	1B/B'	Expression in ossification centers of basioccipital and basisphenoid bones (endochondral).
TS23: vibrissa follicle	Present		1B/B'

Structure	Level	Pattern	Image	Note
TS23: femur	Present	Regionally restricted		Expression in ossification center of long bones including the fibula and femur.
TS23: fibula	Present	Regionally restricted		Expression in ossification center of long bones including the fibula and femur.
TS23: cerebral cortex	Present

Structure	Level	Pattern	Image	Note
TS25: metatarsus	Present	Regionally restricted	1C	Expression in hypertrophic chondrocytes at the site of newly formed ossification center in metatarsal. Expression in cells lining inner layer of perichondrium/periosteum at diaphysis near hypertrophic zone of cartilage.

Structure	Level	Pattern	Image	Note
TS25: femur	Present	Regionally restricted	1D	Expression in hypertrophic chondrocytes at site of newly formed ossification center in the femur. Expression in cells lining inner layer of perichondrium.

Structure	Level	Pattern	Image	Note
TS28: tibia	Present	Regionally restricted	2A/A'	Expression in a subset of hypertrophic cells in secondary ossification center and in deepest layers of growth plate; in osteocytes embedded within trabecular bone matrix near end of growth plate, but not in cells lining trabecular bone.
TS28: perichondrium	Present	Regionally restricted	2A/A'	Expression localized to osteogenic/chondrogenic layer of periosteum/perichondrium lining metaphysis at junction of growth plate and trabecular bone. Expression in osteoblasts within the osteoid lined up along periosteum.
TS28: periosteum	Present	Regionally restricted	2A/A'	Expression localized to osteogenic/chondrogenic layer of periosteum/perichondrium lining metaphysis at junction of growth plate and trabecular bone. Expression in osteoblasts within the osteoid lined up along periosteum.
TS28: hindlimb joint	Present	Regionally restricted	2A/A'	Expression in superficial layer of articular cartilage.
TS28: tendon	Present		2A/A'

Structure	Level	Pattern	Image	Note
TS28: hindlimb long bone	Present	Regionally restricted	3A	Expression in only a subset of hypertrophic cells in the secondary ossification center and in the deepest layers of the growth plate. Higher levels in cells proximal to ossification center relative to cells that are more distal. Expression

Structure	Level	Pattern	Image	Note
TS28: perichondrium	Present	Regionally restricted	3B	In perichondrium at junction of growth plate and trabecular bone, expression localized to cells within osteogenic/chondrogenic layer of perichondrium; no expression in fibrous layer.
TS28: hindlimb long bone	Present	Regionally restricted	3B	Expression in cells in osteoid layer underlying periosteum, as well as hypertrophic cells at transition between growth plate and trabecular bone. Expression in stromal fibroblasts invading the future marrow cavity.

Structure	Level	Pattern	Image	Note
TS28: hindlimb joint	Present	Regionally restricted	3C	Expression in superficial layer of future articular cartilage.

Structure	Level	Pattern	Image	Note
TS28: hindlimb joint	Present	Regionally restricted	4A/A'	Expression in the superficial zone of the articular cartilage.
TS28: hindlimb long bone	Present	Regionally restricted	4A/A'	Expression in subset of hypertrophic cells at transition between articular cartilage and secondary ossification center. periosterium/perichondrium lining metaphysis at junction of growth plate and trabecular bone.