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Gene Expression Data
Assay Details
Assay
Reference: J:77801 Soo K, et al., Twist function is required for the morphogenesis of the cephalic neural tube and the differentiation of the cranial neural crest cells in the mouse embryo. Dev Biol. 2002 Jul 15;247(2):251-70
Assay type: RNA in situ
MGI Accession ID: MGI:5491314
Gene symbol: En1
Gene name: engrailed 1
Probe: En1 probe17
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 2A +/+: embryonic day 8.5 (more )
Note: Also stained for Six3 (assay MGI:5491308); En1 expression indicated by a red arrow.
Structure Level Pattern Image Note
TS13: future midbrain Present 2A +/+
TS13: midbrain-hindbrain junction Present 2A +/+
Specimen 2A -/-: embryonic day 8.5; Twist1tm1Bhr/Twist1tm1Bhr (more )
Note: Also stained for Six3 (assay MGI:5491308); En1 expression indicated by a red arrow.
Structure Level Pattern Image Note
TS13: future midbrain Present 2A -/- Expression expands anteriorly in the mutant.
TS13: midbrain-hindbrain junction Present 2A -/- Expression expands anteriorly in the mutant.
Specimen 2E +/+: embryonic day 9.5 (more )
Note: Also stained for Hesx1 (assay MGI:5491326); En1 expression indicated by a red arrow.
Structure Level Pattern Image Note
TS15: future midbrain Present 2E +/+
TS15: midbrain-hindbrain junction Present 2E +/+
Specimen 2E -/-: embryonic day 9.5; Twist1tm1Bhr/Twist1tm1Bhr (more )
Note: Also stained for Hesx1 (assay MGI:5491326); En1 expression indicated by a red arrow.
Structure Level Pattern Image Note
TS15: future midbrain Present 2E -/- Expression expands anteriorly in the mutant.
TS15: midbrain-hindbrain junction Present 2E -/- Expression expands anteriorly in the mutant.
Specimen 2G +/+: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: midbrain Present 2G +/+
TS17: midbrain-hindbrain junction Present 2G +/+
Specimen 2G -/-: embryonic day 10.5; Twist1tm1Bhr/Twist1tm1Bhr (more )
Structure Level Pattern Image Note
TS17: midbrain Present 2G -/- Expression expands anteriorly in the mutant.
TS17: midbrain-hindbrain junction Present 2G -/- Expression expands anteriorly in the mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory