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Gene Expression Data
Assay Details
Assay
Reference: J:38196 Masuya H, et al., Multigenic control of the localization of the zone of polarizing activity in limb morphogenesis in the mouse. Dev Biol. 1997 Feb 1;182(1):42-51
Assay type: RNA in situ
MGI Accession ID: MGI:5491217
Gene symbol: Gli3
Gene name: GLI-Kruppel family member GLI3
Probe: pGli-3a
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 3A: embryonic day 10.5 (more )
Note: Stage 1 forelimb.
Structure Level Pattern Image Note
TS17: forelimb bud mesenchyme Present Regionally restricted 3A Expressed in the mesenchyme of the anterior portion, being strongest at the anterior margin.
Specimen 3B: embryonic day 11.0 (more )
Note: Stage 2 forelimb.
Structure Level Pattern Image Note
TS18: forelimb bud mesenchyme Present Regionally restricted 3B Expressed in the mesenchyme of the anterior portion, being strongest at the anterior margin.
Specimen 3C: embryonic day 11.5 (more )
Note: Stage 3 forelimb.
Structure Level Pattern Image Note
TS19: forelimb bud mesenchyme Present Regionally restricted 3C Expressed in the mesenchyme of the anterior portion, being strongest at the anterior margin.
Specimen 3D: embryonic day 12.5 (more )
Note: Stage 5 forelimb.
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present Regionally restricted 3D Expression faded toward the posterior side.
Specimen 3E: embryonic day 13.0 (more )
Note: Stage 8 forelimb.
Structure Level Pattern Image Note
TS21: forelimb interdigital region mesenchyme Present 3E
Specimen 3K: embryonic day 10.5 (more )
Note: Stage 0 hindlimb.
Structure Level Pattern Image Note
TS17: hindlimb bud mesenchyme Present Regionally restricted 3K Expressed in the mesenchyme of the anterior portion of the future limb field before the beginning of limb outgrowth.
Specimen 3L: embryonic day 11.0 (more )
Note: Stage 2 hindlimb.
Structure Level Pattern Image Note
TS18: hindlimb bud mesenchyme Present Regionally restricted 3L Expressed in the mesenchyme of the anterior portion, being strongest at the anterior margin.
Specimen 3M: embryonic day 11.5 (more )
Note: Stage 4 hindlimb.
Structure Level Pattern Image Note
TS19: hindlimb bud mesenchyme Present Regionally restricted 3M Expression faded toward the posterior side.
Specimen 3N: embryonic day 12.5 (more )
Note: Stage 7 hindlimb.
Structure Level Pattern Image Note
TS20: hindlimb interdigital region mesenchyme Present 3N
Specimen 4A: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: hindlimb bud Present 4A
Specimen 4B: embryonic day 11.0; Is(In8B2-8B3.1;6C1)1Tshir/Is(In8B2-8B3.1;6C1)1Tshir (more )
Structure Level Pattern Image Note
TS18: hindlimb bud Present 4B No gross alteration of distribution, compared with wild type.
Specimen not shown: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: forelimb bud Present
Specimen not shown: embryonic day 11.0; Is(In8B2-8B3.1;6C1)1Tshir/Is(In8B2-8B3.1;6C1)1Tshir (more )
Structure Level Pattern Image Note
TS18: forelimb bud Present No gross alteration of distribution, compared with wild type.
Specimen not shown: embryonic day 12.0; Is(In8B2-8B3.1;6C1)1Tshir/Is(In8B2-8B3.1;6C1)1Tshir (more )
Structure Level Pattern Image Note
TS20: limb Present No gross alteration of distribution, compared with wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
MGI 6.24 		The Jackson Laboratory