Probe:	Hox 2.4 probe4
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS11: allantois	Present		2A/B

Structure	Level	Pattern	Image	Note
TS11: allantois	Present		2C/D
TS11: primitive streak	Present	Regionally restricted	2C/D	Expression was detected within and along the posterior part of the primitive streak in ectoderm and mesoderm. The rostral boundary of expression was more posterior in the mesoderm than in the ectoderm.

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present	Regionally restricted	2E/F	The rostral limit of expression is in neurectoderm located more anteriorly than the node.

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	2G/H	The rostral expression boundary in the neurectoderm was at the level of the 6th somite.
TS13: trunk unsegmented mesenchyme	Present		2G/H	The rostral boundary of expression in the presomitic mesoderm was at a more posterior position than the 6th somite.

Structure	Level	Pattern	Image	Note
TS15: somite 12	Present		2I/J	Expression in mesoderm extended from somite 12 down to the tail bud.
TS15: tail mesenchyme	Present		2I/J	Expression in mesoderm extended from somite 12 down to the tail bud.
TS15: trunk paraxial mesenchyme	Present		2I/J	Expression in mesoderm extended from somite 12 down to the tail bud.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present	Regionally restricted	2K/L	The rostral boundary of expression in the neurectoderm was in the posterior hindbrain/upper spinal cord.
TS15: future spinal cord	Present	Regionally restricted	2K/L	The rostral boundary of expression in the neurectoderm was in the posterior hindbrain/upper spinal cord.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	2M/N	The rostral boundary of expression in the neurectoderm was in the posterior hindbrain/upper spinal cord at the level of the 6th somite.
TS20: spinal cord	Present	Regionally restricted	2M/N	The rostral boundary of expression in the neurectoderm was in the posterior hindbrain/upper spinal cord at the level of the 6th somite.
TS20: embryo mesenchyme	Present		2M/N

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	2O/P	Expression was dorsally restricted.
TS20: embryo mesenchyme	Ambiguous		2O/P	Expression was doown-regulated in most mesodermal tissues.
TS20: gland	Strong	Regionally restricted	2O/P	Expression was detected in the adrenal gland.
TS20: stomach mesenchyme	Strong		2O/P

Structure	Level	Pattern	Image	Note
TS20: ureter	Present
TS20: metanephros	Present	Regionally restricted		Expression was detected in the kidney tubules.
TS20: male reproductive system	Present	Regionally restricted		Expression was detected in vas deferens and seminal vesicles.

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Strong	Regionally restricted	3A/B	Expression was detected in both ectoderm and mesoderm.

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Strong	Regionally restricted	3C/D	Expression was equally strong in the mesoderm and ectoderm in the posterior part of the streak.

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Strong	Regionally restricted	3E/F	The rostral extermity of the expression domain in the ectoderm was more anterior than in the mesoderm. Expression decreased in the mesoderm of more anterior sections.

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Strong	Regionally restricted	3G/H	Expression remains strong in the streak, but in more anterior sections, the level in mesoderm becomes undetectable at the level of the most rostral positive ectoderm.